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N7653

Sigma-Aldrich

p-Nitrophenyl Phosphate Liquid Substrate System

alkaline phosphatase substrate, liquid

Synonym(s):

4-Nitrophenyl phosphate disodium salt solution, pNPP

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100 ML
$145.00

$145.00

Estimated to ship onApril 11, 2025

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100 ML
$145.00

About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2
CAS Number:
4264-83-9
Molecular Weight:
263.05
MDL number:
MFCD00007319
UNSPSC Code:
12352204
PubChem Substance ID:
24897835
NACRES:
NA.83

$145.00

Estimated to ship onApril 11, 2025

Product Name

p-Nitrophenyl Phosphate Liquid Substrate System, liquid

form

liquid

Quality Level

200

shipped in

wet ice

storage temp.

−20°C

SMILES string

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

General description

The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.[1]

Application

p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase[2] in alkaline phosphatase assay.[3]
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Biochem/physiol Actions

4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP produces p-nitrophenol.[4] It is a common chromogenic substrate for alkaline phosphatase.[5]

Caution

Stable at least one year when stored below 0 °C.

Physical form

Ready-to-use.

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

target_organs

Kidney,Liver,Blood

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCN6783
SLCN6783
SLCL1721
SLCF2308
SLBP4121V

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Mingzhu Chen et al.
European journal of medicinal chemistry, 178, 108-115 (2019-06-09)
As dual regulators, the PTP-1B signaling pathway and α-glucosidase slow glucose release and increase the degree of insulin sensitivity, representing a promising therapeutic target for type 2 diabetes. In this study, we systematically examined the in vivo and in vitro anti-diabetic activities
Cheng-Chi Wang et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(33), 11661-11666 (2008-08-12)
Cancer-associated carbohydrate antigens are often found on the surface of cancer cells. Understanding their roles in cancer progression will lead to the development of new therapeutics and high-sensitivity diagnostics for cancers. Globo H is a member of this family, which
Evaluation of selective laser sintered polyamide/hydroxyapatite composite compositions-in vitro and in vivo
Kamarajan, et al.
International journal of biomedical research, 80(8), 467-474 (2007)
J V Jones et al.
Journal of immunological methods, 118(1), 79-84 (1989-03-10)
We have recently described a substrate amplification system, based on the method of Self, which increases the sensitivity of alkaline phosphatase (AP)-dependent enzyme-linked immunosorbent assays (ELISA) by a factor of 30-50. This increase is achieved by having AP, the primary
Humanization of anti-human insulin receptor antibody for drug targeting across the human blood-brain barrier
Boado RJ, et al.
Biotechnology and Bioengineering, 96(2), 381-391 (2007)
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Questions

1–4 of 4 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. What is the concentration of Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System?

    1 answer

    1. The concentration of this product is considered proprietary.

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  3. What type of application can Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used in?

    1 answer

    1. This product is recommended for ELISA (microwell) procedures and is not recommended for membrane applications.

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  4. How should Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used?

    1 answer

    1. Information on usage is found in our product information sheet (under Documents, above).

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