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N2912

Sigma-Aldrich

Anti-Neurofilament 160/200 antibody, Mouse monoclonal

~2 mg/mL, clone RMdO20, purified from hybridoma cell culture

Synonym(s):

Anti-NEF3

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About This Item

MDL number:
MFCD00164708
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

RMdO20, monoclonal

form

buffered aqueous solution

species reactivity

mouse, rat, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using extracts of rat brain S1 fraction.

isotype

IgG1

UniProt accession no.

P07197

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NEFH(4744) , NEFM(4741)
mouse ... Nefh(380684) , Nefm(18040)
rat ... Nefh(24587) , Nefm(24588)

General description

Neurofilaments are 10 nm in diameter and belong to intermediate filament protein family. Neurofilaments are expressed mainly in cells or tissues of neuronal origin and are crucial for proper radial growth of axons. Monoclonal anti-neurofilament 160/200 antibody can be used in ELISA and immunoblotting. It can also be used in microarray. Mouse anti-neurofilament 160/200 antibody reacts specifically with the non-phosphorylated form of NF-M and NF-H (NF-M/H, approx.160 and 200 kDa) in mouse, rat and human.

Immunogen

purified mid-size rat neurofilament (NF-M) subunit.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal anti-neurofilament 160/200 antibody can be used in immunocytochemistry (diluted 1: 100) and western blotting (diluted 1: 1,000) . It can also be used in immunohistochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kim Braeckman et al.
NeuroImage. Clinical, 21, 101669-101669 (2019-01-20)
Diffusion magnetic resonance imaging biomarkers can provide quantifiable information of the brain tissue after a mild traumatic brain injury (mTBI). However, the commonly applied diffusion tensor imaging (DTI) model is not very specific to changes in the underlying cellular structures.
V M Lee et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 7(11), 3474-3488 (1987-11-01)
A new panel of greater than 300 monoclonal antibodies (mAbs) was prepared to the high, middle, and low Mr rat neurofilament (NF) subunits (NF-H, NF-M and NF-L, respectively). NF proteins were purified both from native, i.e., phosphorylated rat NFs and
Sean P Murphy et al.
Journal of neurochemistry, 129(3), 509-515 (2013-10-24)
The administration of pan histone deacetylase (HDAC) inhibitors reduces ischemic damage to the CNS, both in vitro and in animal models of stroke, via mechanisms which we are beginning to understand. The acetylation of p53 is regulated by Class I
Mizuho Takagi et al.
Journal of toxicologic pathology, 23(2), 111-114 (2010-06-01)
N-nitrosobis(2-hydroxypropyl)amine (BHP) is a well-known carcinogen and induces tumors in various tissues. In the present paper, a case of olfactory neuroblastoma (ONB) induced in a rat by BHP is described. The tumor was observed in one out of 25 male
L Yao et al.
Gene therapy, 20(12), 1149-1157 (2013-07-26)
Functionalized biomaterial scaffolds targeted at improving axonal regeneration by enhancing guided axonal growth provide a promising approach for the repair of spinal cord injury. Collagen neural conduits provide structural guidance for neural tissue regeneration, and in this study it is
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