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MAK221

Sigma-Aldrich

Beta-Lactamase Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

β-Lactamase Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric

storage temp.

−20°C

General description

β-Lactamase (bL; EC 3.5.2.6) is an enzyme first identified in Escherichia coli and has been described as penicillinase. A number of βLs have since been identified from various bacteria. βLs specifically hydrolyze β-lactam rings[1] present in antibiotics such as penicillin, cephalosporins, monobactam, and carbapenem, and confer resistance against these antibiotics.[2]

Suitability

Suitable for the detection of β-lactamase activity in biological samples, food, and fermentation samples.

Principle

The β-Lactamase Activity Assay Kit provides a simple assay for measuring βL activity in βL-secreting bacteria in fermentation media and bacterial cultures. It can also be used to detect βL-secreting bacteria in saliva, urine, and serum of infected mammals and in food samples. βL activity is measured by hydrolyzing a chromogenic cephalosporin called nitrocefin, producing a colorimetric product (A490), proportional to the enzymatic activity present. One unit of β-lactamase is the amount of enzyme required to hydrolyze 1.0 μmole of nitrocefin per minute at pH 7.0 at 25 °C.

Kit Components Only

Product No.
Description
  • β-Lactamase Assay Buffer
  • Nitrocefin, in DMSO
  • Positive Control
  • βL Hydrolysis Buffer

replaced by

Product No.
Description
Pricing

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
8H15K08030
8L01K08030
6G14K08030
5J11K08030
5A17K08030

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Jennifer M Colquhoun et al.
mBio, 12(6), e0313721-e0313721 (2021-12-08)
β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii. In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up
A S Gargis et al.
mSystems, 3(6) (2018-12-24)
Penicillin (PEN) is a low-cost option for anthrax treatment, but naturally occurring resistance has been reported. β-Lactamase expression (bla1, bla2) in Bacillus anthracis is regulated by a sigma factor (SigP) and its cognate anti-sigma factor (RsiP). Mutations leading to truncation
Beta-lactam antibiotics: from antibiosis to resistance and bacteriology.
Kong K F, et al.
Acta Pathologica, Microbiologica et Immunological Scandinavica, 118(1), 1-36 (2010)
Class C β-lactamases operate at the diffusion limit for turnover of their preferred cephalosporin substrates.
Bulychev A and Shahriar M
Antimicrobial Agents and Chemotherapy, 43(7), 1743-1746 (1999)

Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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