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MAK189

Sigma-Aldrich

A-KG Dehydrogenase Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonym(s):

α-KG Dehydrogenase Activity Assay Kit, α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit, AKGDH Assay Kit, Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit, OGDC Activity Assay Kit, Oxoglutarate Dehydrogenase Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; orthopedic diseases; neurological disorders; rheumatological diseases; cancer; cardiovascular diseases

storage temp.

−20°C

Gene Information

human ... OGDH(4967)
mouse ... OGDH(18293)
rat ... OGDH(360975)

General description

α-Ketoglutarate Dehydrogenase (α-KGDH, EC 1.2.4.2) is a highly regulated Krebs cycle enzyme, forms the E1 subunit of the mitochondrial 2-oxoglutarate dehydrogenase complex. It catalyzes the conversion of α-ketoglutarate to succinyl-CoA and produces NADH directly providing electrons for the respiratory chain. Decreased α-KGDH complex activity has been associated with oxidative stress and neurodegenerative disorders such as Alzheimer′s and Parkinson′s diseases.[1]

Application

α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of α-ketoglutarate dehydrogenase in samples.[2][3]

Suitability

Suitable for the detection of α-ketoglutarate dehydrogenase activity in samples such as tissue, cells, and purified mitochondria.

Principle

The α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit provides a simple procedure for measuring α-ketoglutarate dehydrogenase activity in a variety of tissues, cells, and isolated mitochondria. α-KGDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of α-ketoglutarate dehydrogenase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.5 at 37 °C.

Kit Components Only

Product No.
Description
  • KGDH Assay Buffer
  • KGDH Substrate
  • KGDH Developer
  • NADH Standard
  • KGDH Positive Control

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

hcodes

H314,H334

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class

8A - Combustible corrosive hazardous materials

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Certificates of Analysis (COA)

Lot/Batch Number
B1J270678V
B2F200678V
B1D100678V
B8K270678V

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Sirtuin lipoamidase activity is conserved in bacteria as a regulator of metabolic enzyme complexes.
Rowland E A, et al.
mBio, 8(5), e01096-e01017 (2017)
Poldip2 is an oxygen-sensitive protein that controls PDH and αKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer.
Paredes F, et al.
Proceedings of the National Academy of Sciences of the USA, 115(38), 201720693-201720693 (2018)
Alpha-ketoglutarate dehydrogenase: a target and generator of oxidative stress.
Tretter L and Vera A
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 360(1464), 2335-2345 (2005)
Poldip2 is an oxygen-sensitive protein that controls PDH and αKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer.
Paredes F, et al.
Proceedings of the National Academy of Sciences of the USA, 2018, 201720693-201720693 null
Elizabeth A Rowland et al.
mBio, 8(5) (2017-09-14)
Lipoic acid is an essential metabolic cofactor added as a posttranslational modification on several multimeric enzyme complexes. These protein complexes, evolutionarily conserved from bacteria to humans, are core regulators of cellular metabolism. While the multistep enzymatic process of adding lipoyl
View All Related Papers

Questions

  1. What cell types has the MAK189 A-KG Dehydrogenase Activity Assay Kit been tested for?

    1 answer

    1. The MAK189 A-KG Dehydrogenase Activity Assay Kit has been tested for the following:

      Animal tissues: liver, heart, muscle, etc.
      Purified mitochondria
      Cell culture: Adherent or suspension cells. Unfortunately, there is no available data for prokaryotic cells.

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