This is the closest protocol for liver homogenate for use of the kit with cell lysates (presumably cultured cells):
- Lyse cells in 0.5 N NaOH for 30 minutes on a shaking plate followed by a 30-minute incubation at 100 degrees C.
- Clarify the lysate by centrifugation (2 min at 14,000 rpm) and use the clear supernatant for the assay.
- Avoid the use of divalent chelators like EDTA during the preparation.
It indicates that Reagent A contains Trichloroacetic acid (concentration not provided), so it should neutralize the NaOH in the lysate. It is recommended to review the kit's Technical Bulletin regarding the possible need to centrifuge samples that contain proteins.
MAK127
Copper Assay Kit
sufficient for 250 colorimetric tests
Synonym(s):
Copper Test Kit
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1 KIT
$595.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 250 colorimetric tests
detection method
colorimetric
relevant disease(s)
orthopedic diseases; aging/geriatric diseases; hematological disorder; gastrointestinal diseases; neurological disorders; dermatological diseases
storage temp.
2-8°C
Related Categories
General description
Copper is an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin, and collagen. Copper is mainly present in ceruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anemia, hypotonia, and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson′s disease, microcytic hypochromic anemia, and bone disease due to reduced collagen synthesis.
Application
Copper Assay Kit has been used to determine the copper content colorimetrically in samples.[1]
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
Suitability
Suitable for the detection of of copper in biological, environmental, food, and beverage samples. Suitable for studying the effects of compounds on copper metabolism.
Principle
The method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured colorimetrically (359 nm), is directly proportional to copper concentration in the sample. The range of linear detection is 7 μg/dL (1.0 μM) to 300 μg/dL (47 μM).
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
target_organs
Respiratory system
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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How to homogenize the mouse liver, for example, in what buffer, to measure copper content using the MAK127 Copper Assay Kit?
1 answer-
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Does this product solely detect free copper in the solution, or does it also measure bound copper, such as Ceruloplasmin-bound copper?
1 answer-
This kit is not specifically validated for ceruloplasmin-bound copper, But, it is believed that the assay can measure ceruloplasmin-bound copper because it uses a very strong copper chelate. Any other means to dissociate the Cu (such as heating to denature protein) would be helpful, although they may not be necessary.
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