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MAK085

Sigma-Aldrich

Lipid Peroxidation (MDA) Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

MDA Assay, TBARS Assay, Thiobarbituric Acid Reactive Substances Assay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

drug control

regulated under CDSA - not available from Sigma-Aldrich Canada

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases

storage temp.

−20°C

General description

Lipid peroxidation is the degradation of lipids that occurs as a result of oxidative damage and is a useful marker for oxidative stress. Polyunsaturated lipids are susceptible to an oxidative attack, typically by reactive oxygen species, resulting in a well-defined chain reaction with the production of end products such as malondialdehyde (MDA). Lipid peroxidation may contribute to the pathology of many diseases including atherosclerosis, diabetes, and Alzheimer′s.

Application

Lipid peroxidation (MDA) assay kit has been used to determine the levels of malondialdehyde (MDA).[1][2]

Suitability

Suitable for the measurement of malondialdehyde (MDA) in a variety of samples including tissue, cells and plasma

Principle

In this kit, lipid peroxidation is determined by the reaction of MDA with thiobarbituric acid (TBA) to form a colorimetric (532 nm)/fluorometric (λex = 532/λem = 553 nm) product, proportional to the MDA present.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1C

Storage Class

3 - Flammable liquids

flash_point_f

57.2 °F - closed cup

flash_point_c

14 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8L03K07390
8A20K07390
8F15K07390
8H25K07390
8C28K07390

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Maria I Ayuso et al.
ACS chemical neuroscience, 8(10), 2202-2213 (2017-07-22)
There is a need to develop additional effective therapies for ischemic stroke. Nitrones, which were first developed as reactive oxygen species (ROS)-trapping compounds, have been proposed as neuroprotective agents for ischemic stroke, a ROS-related disorder. The previous reported ROS-trapping compound
Aldehyde dehydrogenase 2 protects against oxidative stress associated with pulmonary arterial hypertension.
Xu T, et al.
Redox Biology, 11, 286-296 (2017)
Marco A Perrone et al.
Journal of cardiovascular medicine (Hagerstown, Md.), 20(7), 419-426 (2019-10-09)
Cardiovascular diseases (CVDs) are the most frequent causes of death in the world. Inflammation and oxidative damage contribute significantly to the development of atherosclerosis and CVDs. European Food Safety Authority scientific opinion has acknowledged that hydroxytyrosol (3,4-dihydroxyphenylethanol) and derivatives, contained
Karin Wendin et al.
Foods (Basel, Switzerland), 9(6) (2020-06-21)
To increase the acceptability of insects as food in Western culture, it is essential to develop attractive, high-quality food products. Higher acceptability of insect-based food has been shown if the insects are "invisible". Mincing or chopping the insect material could
Charlotte Berthelot et al.
International journal of phytoremediation, 19(12), 1118-1125 (2017-05-20)
Dark septate endophytes (DSEs) are abundant in stressful environments, including trace element (TE)-enriched soils. However, knowledge about the effects of DSEs on plant growth in such soils is poor compared to the well-known mycorrhizal fungi. The aim of this work
View All Related Papers

Questions

1–7 of 7 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is the MAK085-1KT Lipid Peroxidation (MDA) Assay Kit compatible with tissue samples stored in 4% paraformaldehyde PBS?

    1 answer

    1. The assay has not been tested in PFA-fixed or methanol-fixed samples, and it is not predicted that the kit would work robustly or consistently in these samples.

      Helpful?

  4. Is MAK085 suitable for use on plant samples?

    1 answer

    1. The MAK085 kit has never been tested on plant samples, and thus there is no optimized protocol for plant samples. This kit has only been validated for tissue, cells, serum, or plasma samples. It is possible to adapt the protocol for plant samples and test it, but there is no guarantee that it will work.

      Helpful?

  5. We are planning an MDA assay using mouse tissue. Should I avoid cryopreservation of the samples? How should we store the samples if they cannot be assayed immediately?

    1 answer

    1. Whenever possible, It is recommended to use fresh samples. If sufficient fresh samples are not available, samples may be flash frozen in liquid N2 and stored at -80C. A storage time frame has not been determined. Cryopreservation of samples should be avoided as this may affect the integrity biomolecules and contribute to oxidative stress.

      Helpful?

  6. Can you please send us a protocol how to use MDA Assay kit (MAK085-1KT). We have ordered a kit with lot# 8L03K07390. Thanks

    1 answer

    1. Please see the link below to review the product datasheet which includes the protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/779/633/mak085pis-ms.pdf

      Helpful?

  7. MDA assay kit 구성품 중 TBA(Catalog Number MAK085D)이 4 bottles 있다고 명시되어있는데, 1 bottle 당 몇 g이 들어가있는건가요?

    1 answer

    1. Each bottle contains 250 mg of Thiobarbituric Acid (TBA).

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