This assay is designed for the detection of ascorbic acid and is capable of detecting 0.01-10 nmol of ascorbic acid per assay.
MAK074
Ascorbic Acid Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Vitamin C Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
storage temp.
−20°C
General description
The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.
Suitability
Suitable for use with biological fluids, food samples, and growth media. Proteins present in the sample can interfere with this assay. Protein containing samples are best analyzed using the Ascorbic Acid Assay II (MAK075). Alternatively, samples can be deproteinized using a 10 kDa Molecular Weight Cut-Off Spin Filter.
Principle
In this assay, Ascorbic Acid concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/ λem = 587 nm) product, proportional to the ascorbic acid present.
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Description
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Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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What is the limit of detection for this assay?
1 answer-
Helpful?
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What is the minimum detectable limit for your Ascorbic acid color metric assay kit. What is the accuracy level +/- ppm.
1 answer-
While the absolute minimal limit has not been determined, this kit is intended and the standard curve is set up to detect 0.01-10 nmol of ascorbic acid per assay.
Please see the link below to the product datasheet for further information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/590/655/mak074bul.pdfHelpful?
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