Perchloric acid (PCA) is recommended for deproteinization, but if unavailable, 10 KDa spin filters can be used as an alternative.
MAK054
α-Ketoglutarate Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric
fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
General description
α-Ketoglutarate (α-KG) is a key intermediate in the Tricarboxylic Acid (TCA) Cycle, formed by the oxidative decarboylation of isocitrate by isocitrate dehydrogenase. The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. α-KG is an important nitrogen transporter. This α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples.
Application
Suitability
Suitable for the measurement of a-Ketoglutarate in a variety of biological samples including cell and tissue lysates
Principle
α-KG concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the α-KG present. Typical sensitivities of detection for this kit are 0.01 to 10 nmoles.
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Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
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Is a 10KDa MWCO spin filter a suitable substitute for perchloric acid in the deproteinization step of the Acetyl-CoA Assay Kit (MAK039)?
1 answer-
Helpful?
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この製品は植物（シロイヌナズナ）でも測定可能ですか。
1 answer-
This kit would be suitable for plant samples assuming that the concentration of a-Ketoglutarate present in the tissue lysate is sufficient to enable detection. This α-Ketoglutarate Assay Kit is suitable for the measurement of a-Ketoglutarate in biological samples including cell and tissue lysates. The minimum amount of a-Ketoglutarate used to set up the standard curve is 2 nmole per well. See the link below to review the protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/644/013/mak054bul.pdfHelpful?
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