MAK025
Iron Assay Kit
sufficient for 100 colorimetric tests
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About This Item
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usage
sufficient for 100 colorimetric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric
relevant disease(s)
cancer; hematological disorder
storage temp.
−20°C
General description
Iron is a mineral that plays an essential role in many biological processes, including DNA synthesis, electron transport and oxygen transport. Iron is a transition element that can form a range of oxidation states, the most common being iron II (Fe2+ or ferrous iron) and iron III (Fe3+ or ferric iron). Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions.
Application
Iron assay kit has been used to determine the concentration of Fe2+ in wild type (WT) and Δ ferric uptake regulator (Fur) strains of A. baumannii.
Suitability
Suitable for the measurement of iron concentration in various samples
Principle
In this assay, iron is released by the addition of an acidic buffer. Samples may be tested directly to measure Fe2+ or reduced to measure total iron (Fe2+ and Fe3+). Released iron is reacted with a chromagen resulting in a colorimetric (593 nm) product, proportional to the iron present. The Iron Assay Kit provides a simple convenient means of measuring iron in a variety of biological samples.
Preparation Note
Kit is compatible with serum and tissue samples but not plasma samples.
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Warning
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Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Storage Class
8A - Combustible corrosive hazardous materials
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