The methylcellulose products available are not currently tested for use in spheroid culture. However, a literature search found that item M0512 has been used for culturing spheroids. Here are references for the preparation of methylcellulose in media:
"Generation of Homogenous Three-Dimensional Pancreatic Cancer Cell Spheroids Using an Improved Hanging Drop Technique" by Matthew J. Ware, PhD, et al.
"Methods in Endothelial Cell Biology," pg 56 by Prof. Dr. Hellmut G. Augustin, Department of Vascular Biology & Angiogenesis Research, Tumor Biology Center at the University of Freiburg.
"Rapid and Stable Formation Method of Human Astrocyte Spheroid in a High Viscous Methylcellulose Medium and Its Functional Advantages" by Fumiya Tao et al.
M7027
Methyl cellulose
viscosity: 15 cP, BioReagent, suitable for cell culture
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$87.70
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100 G
$87.70
250 G
$197.00
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.75
Recommended Products
biological source
synthetic (organic)
Quality Level
product line
BioReagent
form
powder
technique(s)
cell culture | mammalian: suitable
viscosity
12-18 cP, 2 % in H2O(20 °C)
solubility
water: 50 mg/mL, clear to hazy
SMILES string
[*]OC[C@H]1O[C@@H](O[C@@H]2[C@@H](CO[*])O[C@@H](O[*])[C@H](O[*])[C@H]2O[*])[C@H](O[*])[C@@H](O[*])[C@@H]1O[*]
InChI key
YLGXILFCIXHCMC-JHGZEJCSSA-N
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General description
Methyl cellulose is obtained by etherification of cellulose. This commercial cellulose ether exhibits amphiphilic properties and original physicochemical properties. Methyl cellulose is enzyme resistant, non-digestible, non-allergic, and non-toxic.
Application
Methyl cellulose has been used:
- as a vehicle for metformin drug during oral gavage
- as a component of mammary epithelial cell growth medium for mammosphere culture
- as a supplement in spheroid 3D co-cultures for spheroid invasion assays using hanging drop protocol
Biochem/physiol Actions
Methyl cellulose finds applications in various industries such as in the food industry it is used as an emulsifier, texturing agent, thickener, and gelling additive. Methyl cellulose is also used in various cosmetics and personal care products such as hair shampoos, hair styling products, liquid soaps, body washes, lotions and creams, and toothpaste to yield their characteristic thick consistency. In addition, it serves as an additive for pharmaceuticals and is used in the production of capsules for nutritional supplements. Methyl cellulose is used as an alternative to glycerol in a cryopreservation medium. It is used along with culture supplements to enhance proliferation and differentiation in multiple or single lineage-committed hematopoietic progenitor cells. This cellulose derivative also has the potential to prevent the loss of adhesive properties and cell functions of various cell types including human skin fibroblasts, human melanoma cells, and mouse lung fibroblasts cultured in suspension.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.
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Which product is suitable for spheroid culture and How can I prepare DMEM media with 0,24% (w/v) for spheroid culture?
1 answer-
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How to practically reach a 1% solution of Methylcellulose in cell culture media that can't be heated excessively?
1 answer-
This product is supplied in powder form. The solubility in water is 50 mg/mL, resulting in a hazy solution. To prepare a 1% Methylcellulose solution in cell culture media from this stock solution, follow these steps:
A 1% solution requires 1 g of solute per 100 mL of solution, equivalent to 10 mg/mL. The stock solution is 50 mg/mL. To achieve this, take 20 mL of the 50 mg/mL stock solution and dilute it to a total volume of 100 mL.Helpful?
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I would like to use methylcellulose in my cell culture media to optimize organoids in suspension. What methylcellulose product would be the most appropriate? And can I dissolve it directly into the cold media?
1 answer-
The methylcellulose products available are not currently tested for use in organoid suspension. However, a previously available product, S3812, was an IMDM-based stem cell culture medium that included a methylcellulose component with a 4000 cps viscosity, at a concentration of 1%. This would correspond to product M0512. The solubility in cold medium has not been determined. Please see the link below to review the general methylcellulose datasheet which includes a viscosity comparison table:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/183/010/m6385pis.pdfHelpful?
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Can the M7027 pass through a 0.2um filter?
1 answer-
Methyl cellulose has a high molecular weight, and the viscosity of methyl cellulose solutions can also impede its ability to pass through such fine filters, as it can clog the filter pores. For sterilization, a 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to a portion of the methylcellulose falling out of solution.
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Does this product need to be sterilized after dissolving for use in cell culture?
1 answer-
Solutions of this material require sterilization for cell culture applications. A 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to a portion of the methylcellulose falling out of the solution.
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Hello sir, I need to know molar mass or molecular weight of this code of Methyl cellulose or tell me how can i calculate can you help me?
1 answer-
The molecular weight is not analyzed on a lot to lot basis. The approximate molecular weight is 88,000 Da with methoxy substitution between 27.5 - 31.5% (weight).
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Can I use this to make 0.5% solution in water which will be used as the solvent to dissolve neratinib and administrate to mice through oral gavage?
1 answer-
This product can be solubilized in water at 50mg/ml which is a 0.5% solution. This product is tested for use in cell cultue, however it has not been tested for in vivo applications. Suitability for in vivo use will need to be validated by the end-user. See the link below for a general product information sheet for Methyl Cellulose. Note product M7140, which is the general research grade of this product. All details for M7140 will apply to M7027.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/785/m0512pis.pdfHelpful?
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Can I use this Methyl cellulose, Product M7027, for cloning or plaque assays?
1 answer-
Probably not. Product No. M7027 has a very low viscosity (viscosity 15 cP, 2% solution in water) and is used for thickening culture media. Product No. M0387 (viscosity 1,500 cP, 2% solution in water) is recommended for plaque assays, and Product No. M0512 (viscosity 4,000 cP, 2% solution in water) is recommended for cloning or colony growth assays.
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How do I store a stock solution of Methyl cellulose, Product M7027?
1 answer-
A sterile solution of methylcellulose can be stored at room temperature for up to 1 year.
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Is Methyl cellulose, Product M7027, the same as Methocel?
1 answer-
Methocel is a brand name and is a synonym for methylcellulose.
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