5-10 μg/mL (Suggested working concentration to compete out the binding of c-Myc)
technique(s)
protein extraction: suitable
storage temp.
−20°C
Amino Acid Sequence
Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu
General description
The c-Myc Peptide is a synthetic peptide with an amino acid sequence that corresponds to the amino acids 410-419 of the C-terminal of human c-myc.
Application
Useful for displacement of c-Myc-tagged fusion proteins bound to anti-c-Myc antibodies in immunoassays. The successful inhibition of antibody binding by c-Myc peptide demonstrates binding is specific.
Other Notes
Amino acid sequence is corresponds to amino acids 410-419 of the C-terminal of human c-Myc.
Lyophilized from 0.1% TFA in H2O
Preparation Note
Dissolve in water to a final concentration of 5 mg/mL.
PAX8-PPARG fusion protein (PPFP) results from a t(2;3)(q13;p25) chromosomal translocation, is found in 30% of follicular thyroid carcinomas, and demonstrates oncogenic capacity in transgenic mice. A PPARG ligand, pioglitazone, is highly therapeutic in mice with PPFP thyroid cancer. However, only
The molecular basis of higher regenerative capacity of cold-blooded animals comparing to warm-blooded ones is poorly understood. Although this difference in regenerative capacities is commonly thought to be a result of restructuring of the same regulatory gene network, we hypothesized
Biochimica et biophysica acta, 1783(8), 1536-1543 (2008-04-03)
Hexose-6-phosphate dehydrogenase (H6PDH) has been shown to stimulate 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1)-dependent local regeneration of active glucocorticoids. Here, we show that coexpression with H6PDH results in a dramatic shift from 11beta-HSD1 oxidase to reductase activity without affecting the activity
Maintaining the optimal performance of cell processes and organelles is the task of auto-regulatory systems. Here we describe an auto-regulatory device that helps to maintain homeostasis of the endoplasmic reticulum (ER) by adjusting the secretory flux to the cargo load.
Survival of cancer cells in the harsh tumor microenvironment, characterized by oxygen and glucose deprivation, requires rapid initiation of cytoprotective measures. Metabolites whose levels change during stress are ideal signaling cues, particularly if used in post-translational modifications of stress-responsive signal
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.