G6795
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal
clone GSN24, purified from hybridoma cell culture
Synonym(s):
Anti-GFP
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.56
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GSN24, monoclonal
form
buffered aqueous solution
mol wt
antigen 27 kDa
concentration
~2 mg/mL
technique(s)
western blot: 1-2 μg/mL using GFP fusion proteins expressed in extracts of transfected cells
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
2-8°C
target post-translational modification
unmodified
General description
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma GSN24 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice.
Specificity
The antibody reacts specifically with GFP fusion proteins.
Application
Anti-Green Fluorescent Protein (GFP), N-terminal antibody, Mouse monoclonal has been used in: western blotting, enzyme linked immunosorbent assay (ELISA), immunofluorescence labeling.
Biochem/physiol Actions
Green Fluorescent Protein (GFP) is a unique tool in cellular and molecular biology research to monitor gene expression and protein localization in living cells. In the jellyfish, A. victoria, GFP transduces the excitation energy resulting from emission of blue light of the photoprotein aequorin and reemits it as green light.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
related product
Product No.
Description
Pricing
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Marta Ukleja et al.
Nature communications, 7, 10433-10433 (2016-01-26)
CCR4-NOT is a large protein complex present both in cytoplasm and the nucleus of eukaryotic cells. Although it is involved in a variety of distinct processes related to expression of genetic information such as poly(A) tail shortening, transcription regulation, nuclear
Diggin? on U (biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates
Rubel CE, et al.
Cell Biochemistry and Biophysics, 67(1), 127-138 (2013)
Design of inducible expression vectors for improved protein production in Ralstonia eutropha H16 derived host strains
Gruber S, et al.
Journal of Biotechnology, 235(8), 92-99 (2016)
Matthias Wiemer et al.
F1000Research, 3, 230-230 (2014-12-19)
The degradation of damaged proteins is an important vital function especially during aging and stress. The ubiquitin proteasome system is one of the major cellular machineries for protein degradation. Health and longevity are associated with high proteasome activity. To demonstrate
GFP loss-of-function mutations in Arabidopsis thaliana
Fu JL, et al.
G3 (Bethesda, Md.), 5(9), 1849-1855 (2015)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service