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D8187

Sigma-Aldrich

JumpStart™ REDTaq® DNA Polymerase

Hot-start Taq enzyme with inert dye, 10X buffer included

Synonym(s):

Hot start DNA polymerase, Hot start Taq

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About This Item

MDL number:
MFCD00166026
UNSPSC Code:
12352204
NACRES:
NA.55

form

liquid

usage

sufficient for 250 reactions
 sufficient for 2500 reactions
 sufficient for 50 reactions

feature

dNTPs included: no
hotstart

concentration

1 unit/μL

technique(s)

PCR: suitable

color

red

input

purified DNA

suitability

suitable for PCR

shipped in

wet ice

storage temp.

−20°C

General description

JumpStart™ REDTaq® DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.

Application

JumpStart™ REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) for the amplification of:
  • insulin-enterotoxin ricin fusion gene (INS-RTB)
  • endothelial cells DNA derived from reverse transcribed RNA
  • leg genomic DNA from cricket flies
  • mitochondrial gene by conventional PCR
JumpStart™ REDTaq® DNA Polymerase is also suitable for DNA methylation analysis.

Features and Benefits

  • Reduces non-specific amplification
  • Increased target yield and specificity
  • Higher the amplification irrespective of the target concentration
  • Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
  • Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
  • Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
  • Assembled PCR reactions can be placed at room temperature for up to 2 hours

Packaging

The enzyme is provided with an optimized 10× reaction buffer.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Other Notes

View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

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pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

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Contributions of VEGF to age-dependent transmural gradients in contractile protein expression in ovine carotid arteries
Butler SM, et al.
American Journal of Physiology. Cell Physiology, 301(3), C653-C666 (2011)
Sarah D Burnett et al.
Journal of toxicology and environmental health. Part A, 84(24), 1020-1039 (2021-08-25)
Inter-species differences in toxicodynamics are often a critical source of uncertainty in safety evaluations and typically dealt with using default adjustment factors. In vitro studies that use cells from different species demonstrated some success for estimating the relationships between life
Candice L Brinkmeyer-Langford et al.
BMC genomics, 10, 182-182 (2009-04-28)
The cattle MHC is termed the bovine leukocyte antigen (BoLA) and, along with the MHCs of other ruminants, is unique in its genomic organization. Consequently, correct and reliable gene maps and sequence information are critical to the study of the
Billions and billions sold: pet-feeder crickets (Orthoptera: Gryllidae), commercial cricket farms, an epizootic densovirus, and government regulations make for a potential disaster
Weissman DB, et al.
Zootaxa, 3504(1), 67-88 (2012)
Expression of a ricin toxin B subunit: insulin fusion protein in edible plant tissues
Carter JE, et al.
Molecular Biotechnology, 44(2), 90-100 (2010)
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Articles

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Hot Start PCR

The purpose of Hot Start PCR is to inhibit the PCR reaction in order to reduce nonspecific amplification, prevent the formation of primer dimers, and increase product yields.

Protocols

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Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

End-Point PCR: Antibody-Mediated Hot Start PCR Protocol with Enhanced Specificity and Yield

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Antibody-Enzyme Mediated Hot Start PCR Protocol

JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.

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