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C7901

Sigma-Aldrich

Chelex® 100 sodium form

50-100 mesh (dry)

Synonym(s):

Iminodiacetic Acid Resin

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About This Item

CAS Number:
11139-85-8
MDL number:
MFCD00163980
UNSPSC Code:
23151817
NACRES:
NA.56

form

beads

matrix

1% cross-linked polystyrene
 cross-linked polystyrene beads

matrix active group

iminodiacetic acid (sodium form)
paired iminodiacetate groups

matrix attachment

through methyl group to aromatic rings

particle size

50-100 mesh (dry)

pH

4-14

capacity

≥0.3 meq/mL binding capacity
 0.6 meq/g binding capacity (heavy metal ions)

application

Chelex is used in affinity chromatography, protein chromatography, chelating resins, affinity resins and core bioreagents.
Chelex® 100 sodium form has been used:
  • for the extraction of DNA from bee samples
  • to remove from cigarette smoke extract (CSE) and study the effects of hepcidin expression in the murine lungs
  • to extract DNA from bacterial cultures

Biochem/physiol Actions

Chelex® 100 is a styrene-divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. It is considered a weakly acidic resin. Chelex acts as a chelating resin that is used in DNA extraction from forensic samples, such as hair, bloodstain cards, and buccal swabs.

Other Notes

Product is autoclavable in sodium form. It has a maximum operating temperature of 75°C and is not soluble in water.

Legal Information

Chelex is a registered trademark of Bio-Rad Laboratories, Inc.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Food Control, 20(8), 733-738 (2009)
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