C2885

CHES

≥99.0% (titration)

• Synonym(s): 2-(Cyclohexylamino)ethanesulfonic acid
• Empirical Formula (Hill Notation): C₈H₁₇NO₃S
• CAS Number: 103-47-9
• Molecular Weight: 207.29
• Beilstein/REAXYS Number: 2967601
• EC Number: 203-115-6
• MDL number: MFCD00003835
• UNSPSC Code: 12161700
• PubChem Substance ID: 24892541
• NACRES: NA.25

Properties

• assay: ≥99.0% (titration)
• form: crystalline
• pH: 3.0-5.0 (103.6 g/L)
• useful pH range: 8.6-10.0
• pKa (25 °C): 9.3
• solubility: water: 0.6 g/25 mL, clear, colorless
• application(s): diagnostic assay manufacturing
• SMILES string: OS(=O)(=O)CCNC1CCCCC1
• InChI: 1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)
• InChI key: MKWKNSIESPFAQN-UHFFFAOYSA-N

Description

General description

CHES (2-(N-cyclohexylamino)ethanesulphonic acid) is regarded as Good′s buffers for its self-buffering and biocompatible feature. It is used in protein stabilization and does not interact with metals.

Application

CHES has been used:

• as a buffer in mannanase activity assay of antarctic fungal isolates
• as a buffer for DNA digestion and labeling of 2′-deoxyadenosine 3′-monophosphate
• as a component of Wilson and Horne extraction buffer for characterization of rat plasma enzymes

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves