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A4284

Sigma-Aldrich

L-Asparagine monohydrate

suitable for cell culture, BioReagent

Synonym(s):

(S)-(+)-2-Aminosuccinamic acid, (S)-2-Aminosuccinic acid 4-amide, L-Aspartic acid 4-amide

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About This Item

Linear Formula:
NH2COCH2CH(NH2)COOH · H2O
CAS Number:
Molecular Weight:
150.13
Beilstein/REAXYS Number:
1723527
EC Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.75

biological source

synthetic

product line

BioReagent

assay

≥99% (TLC)

form

powder

technique(s)

cell culture | mammalian: suitable

color

white to off-white

mp

233-235 °C (lit.)

SMILES string

[H]O[H].N[C@@H](CC(N)=O)C(O)=O

InChI

1S/C4H8N2O3.H2O/c5-2(4(8)9)1-3(6)7;/h2H,1,5H2,(H2,6,7)(H,8,9);1H2/t2-;/m0./s1

InChI key

RBMGJIZCEWRQES-DKWTVANSSA-N

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Application

L-Asparagine monohydrate has been used:
  • as a media supplement to culture Black Mexican Sweet (BMS) cells
  • to prepare Adams III media to supplement C+Y medium for culturing Streptococcus pneumoniae
  • in an asparagine-rich diet to study the E0771 syngeneic breast tumor growth and survival in mice

Biochem/physiol Actions

L-Asparagine (L-Asp) is a nonessential amino acid involved in the synthesis of critical cellular proteins of human cells. It is synthesized from oxaloacetic acid. L-Asp is used as a growth supplement for tumor cells in in vitro conditions.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Cell wall is a complex biopolymer on the surface of all Gram-positive bacteria. During infection, cell wall is recognized by the innate immune receptor Toll-like receptor 2 causing intense inflammation and tissue damage. In animal models, cell wall traffics from

Protocols

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

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