The emission is at 570 nm when dissolved in water.
83200
Pyronin Y
for microscopy (Bot., Fl., Hist.)
Synonym(s):
Pyronin G, Pyronin J
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5 G
$189.00
About This Item
Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
Molecular Weight:
302.80
Colour Index Number:
45005
Beilstein/REAXYS Number:
3795789
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Recommended Products
grade
for microscopy (Bot., Fl., Hist.)
Quality Level
mp
250-260 °C (lit.)
solubility
water: 0.01 g/10 mL, red
εmax
≥1650 at 543-553 nm in 50% ethanol
≥650 at 249-259 nm in 50% ethanol
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C
InChI
1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
InChI key
INCIMLINXXICKS-UHFFFAOYSA-M
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Biochem/physiol Actions
Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.[1]
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the emission wavelength of Pyronin Y, Product 83200?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solubility of Pyronin Y, Product 83200, in water and ethanol?
1 answer-
Solubility is not tested at Sigma Aldrich, but the literature reports solubility up to 9% (i.e., 90 mg/mL) in water and 0.6% (6 mg/mL) in ethanol.
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Is there a prepared solution of Methyl Green and Pyronin Y for the demonstration of plasma cells and RNA in tissue sections?
1 answer-
Yes, product HT70116 is available in pre-made form. The product is approved for in vitro diagnostic (IVD) use.
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Has Pyronin Y, Product 83200, been use-tested for the demonstration of RNA?
1 answer-
No. There are, however, two other Pyronin products from Sigma-Aldrich which should be suitable. Product No. P9172 has been used to stain RNA in polyacrylamide gels. Product No. 213519 was submitted to the Biological Stain Commission for certification. Testing was performed by the Biological Stain Commission to ensure the Pyronin Y stained RNA in tissue sections.
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