Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

76220

Sigma-Aldrich

Papain from Carica papaya

powder, ≥3 U/mg

Synonym(s):

Papain powder

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9001-73-4
Enzyme Commission number:
3.4.22.2 (BRENDA, IUBMB)
EC Number:
232-627-2
MDL number:
MFCD00131791
UNSPSC Code:
12352204
eCl@ss:
42030311
NACRES:
NA.54

biological source

papaya

form

powder

specific activity

≥3 U/mg

mol wt

Mr ~23000

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Application

Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.

Biochem/physiol Actions

Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C

Other Notes

An important cysteine protease ; Peptide synthesis

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Review: novel cysteine proteases of the papain family.
F Bühling et al.
Advances in experimental medicine and biology, 477, 241-254 (2000-06-13)
Catalytic mechanism in papain family of cysteine peptidases.
A C Storer et al.
Methods in enzymology, 244, 486-500 (1994-01-01)
M Y Gololobov et al.
Biotechnology and bioengineering, 40(3), 432-436 (1992-07-01)
The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters alpha and beta for papain-catalyzed synthesis of Mal-L-Phe-L-Ala-LLeuNH(2) from Mal-L-Phe-L-AlaOMe and L-LeuNH(2). The
Adrienne McMahon
Journal of AOAC International, 99(1), 223-241 (2016-02-10)
The main objective of the AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) project is to establish international consensus methods for infant formula and adult nutritionals, which will benefit intermarket supply and dispute resolution. A collaborative study was
Carolina Gomis Perez et al.
Science advances, 8(1), eabl4411-eabl4411 (2022-01-06)
Many cellular activities, such as cell migration, cell division, phagocytosis, and exo-endocytosis, generate and are regulated by membrane tension gradients. Membrane tension gradients drive membrane flows, but there is controversy over how rapidly plasma membrane flow can relax tension gradients.
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service