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19160

Sigma-Aldrich

SOD Assay Kit

SOD Assay Kit
2 of 2 reviewers received a sample product or took part in a promotion

sufficient for 500 tests

Synonym(s):

Superoxide dismutase Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
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usage

sufficient for 500 tests

Quality Level

100

availability

not available in USA

technique(s)

UV/Vis spectroscopy: suitable

suitability

corresponds for UV test (WST reagent, WST formazan)
use tested

storage temp.

2-8°C

General description

The kit contains all reagents and solutions required for determining superoxide dismutase activity in an indirect assay method based on xanthine oxidase and a novel color reagent. The chemical and biochemical properties of the color reagent used in this kit guarantee a very convenient application and linearity of test results over a broad range.

Application

SOD Assay Kit has been used for the determination of superoxide dismutase activity in mammalian cells.[1][2][3]

Biochem/physiol Actions

Superoxide dismutases are enzymes responsible for the elimination of superoxide radical (O2-), thereby protecting cells from free radicals-associated damages.[4] They catalyze the dismutation of two superoxide radicals to hydrogen peroxide and oxygen.[5]

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL5235
BCCL5234
BCCK6790
BCCK6791
BCCH9840

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Superoxide dismutase as a target for the selective killing of cancer cells.
Huang P et al.
Nature, 407, 390-390 (2000)
Atg7 Knockdown Augments Concanavalin A-Induced Acute Hepatitis through an ROS-Mediated p38/MAPK Pathway.
Zhuang Y et al.
PLoS ONE, 11, e0149754-e0149754 (2016)
Extracellular superoxide dismutase in biology and medicine.
Fattman CL et al.
Free Radical Biology & Medicine, 35, 236-236 (2003)
Hosam O Elansary et al.
Frontiers in plant science, 8, 830-830 (2017-07-01)
The negative effects of the ongoing climate change include unusual prolonged droughts and increased salinity pressures on the agricultural lands. Consequently, crops are facing unprecedented environmental pressure, and this calls for more research toward controlling such major stresses. The current
Youngjin Park et al.
Fish & shellfish immunology, 59, 351-357 (2016-11-08)
An 8-week feeding trial was conducted to evaluate the effects of dietary probiotics on growth performance and non-specific immune responses in starry flounder, Platichthys stellatus. Fish averaging 46.5 ± 0.65 g (mean ± SD) were fed one of the six experimental diets; one control (Cont)
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Questions

1–10 of 14 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Hello, Please what is the quantity of cell sample is required for use in this kit ?

    1 answer

    1. The recommended number of cells is 9x10*6 for adherent lines. Leukocytes have been tested successfully at 1.2x10*7 cells. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/254/301/19160dat.pdf

      Helpful?

  4. Can we use that kit without having SOD? And the shiping can be done at room temperature and put in fridge after reception?

    1 answer

    1. The superoxide dismutase (SOD) is required for preparation of an inhibition curve. This is a SOD inhibitory assay kit. See SOD product S7571, which is suggested for use with this kit. This kit is shipped on wet ice to preserve the quality of the transit. Upon receipt, the item should be stored at 2-8°C. If the interest is in calculating SOD activity in a sample, product CS0009 may be a kit of interest. See the links below to review additional information.

      S7571, Superoxide Dismutase
      https://www.sigmaaldrich.com/product/sigma/s7571

      19160 SOD Assay Kit protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/254/301/19160dat.pdf

      CS0009 SOD Activity Assay Kit
      https://www.sigmaaldrich.com/product/sigma/cs0009

      Helpful?

  5. Hello, Please what is the quantity of tissue sample is required for use in this kit

    1 answer

    1. This product is not routinely tested for use with solid tissue samples. However, the following general preparation method for 100 mg of tissue is recommended:
      1. Rinse the tissue with saline solution to remove residual blood, then gently dab the tissue with paper towels before proceeding to weigh it.
      2. Add 400-900 ml of sucrose buffer (0.25 M sucrose, 10 mM Tris, 1 mM EDTA, pH 7.4) and use a Teflon homogenizer to process the sample. If needed, subject the homogenized sample to sonication on an ice bath (60W with 0.5 second intervals for 15 min).
      3. Centrifuge the homogenized sample at 10,000 g for 60 min at 4ºC then carefully transfer the resulting supernatant to a new tube for measurement.

      Helpful?

  6. What is the SOD concentration?

    1 answer

    1. As per the protocol of 19160: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/254/301/19160dat.pdf, the kit doesn't include SOD standard, that is to say, the SOD standard needs to be purchased separately. As shown on page 2 of the protocol, if assay monitoring is required, the SOD standard should be diluted in the range of 0.001-200 U/mL, indicating the detection range of the kit is 0.001-200 U/mL.

      Helpful?

  7. Where can we find the protocol for analysis? Online

    1 answer

    1. The protocol can be located towards the bottom of the product detail page, under "Data Sheets".
      Please see this copy of the data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/254/301/19160dat.pdf.

      Helpful?

  8. When using Product 19160, SOD Assay Kit, how do I prepare a Superoxide Dismutase (SOD) working solution?

    1 answer

    1. Dilute SOD with Dilution Buffer to prepare SOD standard Solutions if necessary for assay monitoring as follows:  200 U/mL, 100 U/mL, 50 U/mL, 20 U/mL, 10 U/mL, 5 U/mL, 1 U/mL, 0.1 U/mL, 0.05 U/mL, 0.01 U/mL, and 0.001 U/mL.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. How do I prepare a WST working solution for Product 19160, SOD Assay Kit (Superoxide dismutase)?

    1 answer

    1. Dilute 1mL of WST Solution with 19mL of Buffer Solution.

      Helpful?

1–10 of 14 Questions  

Reviews

2 of 2 reviewers received a sample product or took part in a promotion

Active Filters

  1. Padova
    • Review 1
    • Votes 10
    1 out of 5 stars.

    The product works, but the protocol is not clear at all!

    Translate with Google

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for taking the time to leave a review! We are sorry to hear that your experience with the protocol for this assay kit did not meet your expectations. If you have a moment, please contact us by visiting https://www.sigmaaldrich.com/support/customer-support and submit a Product Technical Inquiry so that we can assist you. We look forward to hearing from you soon!

  2. 1 Ratings-Only Review

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