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16016

Millipore

BRILA MUG Broth

suitable for microbiology, NutriSelect® Basic

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

brilliant green, 0.0133 g/L
lactose, 10 g/L
4-methylumbelliferyl-β-D-glucuronide, 0.1 g/L
ox-bile (dried), 20 g/L
L-tryptophan, 1 g/L

manufacturer/tradename

NutriSelect® Basic

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Citrobacter spp.
selective and differential for Escherichia coli
selective and differential for Lactobacillus spp.
selective and differential for Staphylococcus spp.
selective and differential for coliforms

Application

In the detection of E. coli and coliforms. Bile and brilliant green extensively inhibit the growth of accompanying flora, in particular gram-positive microorganisms. The presence of E. coli results in fluorescence in the UV. A positive indole test and possibly gas formation from lactose fermentation provide confirmation.
The broth can be used in conjunction with the MPN method for E. coli and coliform enumeration in the water of bathing areas.

Preparation Note

Dissolve 41 g in 1 litre distilled water and pour into tubesfitted with Durham tubes if necessary. Autoclave at 121°C.

Other Notes

Screening of bathing-water for coliforms according to EC directive

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Environment
GHS09

hcodes

H411

Hazard Classifications

Aquatic Chronic 2

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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G Havemeister et al.
Zentralblatt fur Hygiene und Umweltmedizin = International journal of hygiene and environmental medicine, 191(5-6), 523-538 (1991-05-01)
During the summer season of 1989 about 222 samples of bathing water (Northsea, Baltic Sea and inland waters) were investigated, i.e. 2 times 1998 dilution tubes (1501 positive) were tested. Results with BRILA-MUG and lactose-bouillon were compared and additional 97

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