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Millipore

Oxidase Reagent according to Gaby-Hadley A

suitable for microbiology

Synonym(s):

Gaby-Hadley Reagent A

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About This Item

UNSPSC Code:
41171621
NACRES:
NA.85

agency

according to ISO 9308-1:2014

Quality Level

200

product line

BioChemika

shelf life

limited shelf life, expiry date on the label

composition

ethanol (95%), 100 mL
α-naphtol, 1 g

packaging

pkg of 100 mL

storage condition

(Tightly closed. Dry.)

technique(s)

microbe id | specific enzyme detection: suitable

antibiotic activity spectrum

Gram-negative bacteria

application(s)

agriculture
clinical testing
environmental
food and beverages
pharmaceutical
microbiology

suitability

Plesiomonas spp.
Aeromonas spp.
Neisseria spp.
Pseudomonas spp.

General description

Oxidase Reagent A, according to Gaby-Hadley, is a commonly used laboratory reagent employed in microbiology for the detection of bacteria that produce cytochrome c oxidase. The reagent is designed to identify the presence of this enzyme, which plays a critical role in the electron transport chain and is indicative of the respiratory metabolism in bacteria.The Gaby-Hadley reagent A contains alpha-naphthol as the active component in the reagent. When an organism produces cytochrome c oxidase, the reagent undergoes a color change reaction, turning from colorless to blue or purple. This color change is a positive result, indicating the presence of cytochrome c oxidase activity in the tested organism.

Application

Oxidase Reagent A, according to Gaby-Hadley, is a commonly used laboratory technique in industrial microbiology to differentiate between bacterial strains based on their ability to produce cytochrome c oxidase. The Gaby-Hadley A reagent provides a simple and efficient way to identify oxidase-positive bacteria, such as Pseudomonas, Vibrio, and some Neisseria species, while producing negative results for organisms that lack cytochrome c oxidase activity. in the food, beverage, and pharmaceutical industries.

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

57.2 °F

flash_point_c

14 °C

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCJ4476
BCCG1740
BCCC2541
BCBW8148
BCBT9088

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Articles

Vibrio Detection

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater.

Differentiation of Escherichia coli from coliforms

There are many other methods of detection to indicate the presence of E. coli. Review common tests and biochemical reactions for this contaminant.

Campylobacter Detection and Identification

Specific agars and broths support detection and cultivation of Campylobacter, requiring complex media with essential supplements.

Media for Staphylococcus Aureus Detection

Chromogenic media enable the selective detection of S. aureus, which produce bluish-green colonies that are clearly differentiated from other species.

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Protocols

Enterobacteriaceae in Food

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

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