XGAL-RO

X-Gal

solubility: in DMF, soluble, solubility: in methanol, soluble, storage temp.:2-8°C

• Synonym(s): 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside, bromo-4-chloro-3-indolyl-β-d-galactopyranoside, 5-, 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
• Empirical Formula (Hill Notation): C₁₄H₁₅BrClNO₆
• CAS Number: 7240-90-6
• Molecular Weight: 408.63
• Beilstein/REAXYS Number: 1402009
• MDL number: MFCD00005666
• UNSPSC Code: 12352204
• PubChem Substance ID: 329830810
• NACRES: NA.28

Properties

• description: 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside
• Quality Level: 100
• assay: 99%
• form: solid
• mol wt: 408.63 g/mol
• packaging: pkg of 1 g (10745740001); pkg of 100 mg (03117073001); pkg of 100 mg (11680293001); pkg of 2.5 g (10703729001); pkg of 250 mg (10651745001)
• manufacturer/tradename: Roche
• concentration: 20 mg/mL
• technique(s): activity assay: suitable
• color: white
• solubility: DMF: soluble; methanol: soluble
• application(s): genomic analysis; life science and biopharma
• storage temp.: 2-8°C
• SMILES string: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
• Inchi Key: OPIFSICVWOWJMJ-AEOCFKNESA-N

Description

General description

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.

Application

X-Gal has been used for immunohistocytochemistry applications. It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

Quality

Chromatographically homogeneous

Physical form

Crystals, white powder

Preparation Note

Working solution: Stock solution:

• 20 mg/ml in dimethyl formamide (DMF), clear, colorless solution
• A_{590 nm}: 0.01

Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable