1.00072

GranuCult^® plus Bile Aesculin Azide Agar

ISO 7899-2, for Streptococcus spp., for Enterococcus spp., suitable for microbiology

• Synonym(s): Streptococcus selective agar, BEA agar, Bile Aesculin Agar
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• agency: ISO 7899-2
• Quality Level: 200
• sterility: non-sterile
• form: granular
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• storage condition: protect from light (Keep tightly closed and dry)
• technique(s): microbe id | specific enzyme detection: suitable; microbiological culture: suitable
• color: yellow
• pH: 6.9-7.3 (25 °C, 54.85 g/L in H₂O, after autoclaving)
• solubility: 54.85 g/L
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Enterococcus spp.; Streptococcus spp.

Description

General description

GranuCult^® plus Bile Esculin Azide Agar is a selective medium used for isolation and presumptive identification of fecal Streptococci. The presence of intestinal enterococci serves as an indicator of fecal contamination. BEA agar is employed as a confirmation and enumeration of medium for typical isolation on the primary isolation Membrane Enterococcus Selective Agar (1.05262 and 1.05289). Enterococci and some species of the genus Streptococcus namely S. bovis and S. equines can reproduce normally in this medium. Esculin hydrolysis and bile tolerances are regarded as reliable characteristics of enterococci. Intestinal enterococci hydrolyze the glycoside esculin to give dextrose and esculetin, which forms an olive green to black complex with iron (III) ions.Enterococci are bile tolerant. Bile salts exhibit the growth of numerous accompanying bacteria. The concentration of sodium azide in the medium inhibits the growth of Gram-negative microbial flora while sparing the enterococci.

Application

GranuCult^® plus Bile Aesculin Azide Agar is suitable for the detection and enumeration of intestinal enterococci (fecal streptococci).

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): green-yellow irridescent
pH-value (25 °C): 6.9 - 7.3
Recovery on test medium (Enterococcus faecium ATCC 882): ≥ 60 %
Recovery on test medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 70 %
Recovery on test medium (Enterococcus durans ATCC 6056): ≥ 50 %
Recovery on test medium (Enterococcus hirae ATCC 8043 (WDCM 00089)): ≥ 60 %
Recovery on test medium (Listeria monocytogenes ATCC 19118): ≤ 0.01 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 0.01 %
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): fair
Colony colour (Enterococcus faecium ATCC 882): black
Colony colour (Enterococcus faecalis ATCC 19433 (WDCM 00009)): black
Colony colour (Enterococcus durans ATCC 6056): black
Colony colour (Enterococcus hirae ATCC 8043 (WDCM 00089)): black
Colony colour (Listeria monocytogenes ATCC 19118): colourless
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): colourless
Colony colour (Staphylococcus aureus ATCC 25923 (WDCM 00034)): colourless
Incubation: 24 hrs.; 35°C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3