High molecular weight branched PEI is commonly used in gene delivery due to its high transfection efficiency in a broad range of cells. Branched PEI has been used to successfully deliver genetic material both in vitro and in vivo. N-Acetylation of PEI could lower its toxicity. At low degree of substitution (below 25%) resulted in moderate improvement of transfection activity.
Polyethylenimine (PEI) is a highly effective gene delivery vector, but because it is an off-the shelf material, its properties may not be optimal. To investigate the effects of the protonation properties of the polymer, we generated PEI derivatives by acetylating
We previously reported that gene delivery efficiency of 25-kDa, branched polyethylenimine (PEI) increased upon acetylation of up to 43% of the primary amines with acetic anhydride. In the present work, we investigated the effects of further increasing the degree of
Polymer carriers like PEI which proved their efficiency in DNA delivery were found to be far less effective for the applications with siRNA. In the current study, we generated a number of nontoxic derivates of branched PEI through modification of
CRISPR/Cas9 delivery via nonviral nanoparticles shows promising advancements for gene editing in disease treatment.
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