SAB4200682

Anti-Human IgG (Fc specific) antibody, Mouse monoclonal

clone GG-7, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 300
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: GG-7, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): ELISA: suitable; immunofluorescence: suitable; indirect ELISA: 0.25-0.5 μg/mL using 5μg/mL human IgG for coating.
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Human IgG (Fc specific) (mouse IgG1 isotype) is derived from the GG-7 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. IgGs are the most common Immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid, and peritoneal fluid. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), they consist of a variable Fab fragment (which includes the antibody recognition site), and a conserved Fc fragment.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads

Application

Monoclonal Anti-Human IgG (Fc specific) antibody has been used in:

• ELISA
• Imprint Immunofixation (IIF)
• Immunofluorometric Assay (IFMA)
• Hemagglutination (HA)
• Hemagglutination Inhibition (HAI)
• Particle Counting Immunoassay (PACIA)
• detection of cytoplasmic IgG

Biochem/physiol Actions

The crystallizable fragment (Fc) of IgG has various important functions such as complement fixation, site for rheumatoid factor (autoantibody directed to Fc) attachment, passage through the placental membrane and protein A binding. IgG deficiencies are often associated with various diseases.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable