SAB4200454

Anti-FUS antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-ALS6, Anti-CHOP, Anti-FUS-CHOP, Anti-FUS1, Anti-TLS, Anti-TLS/CHOP, Anti-hnRNP-P2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~68 kDa
• species reactivity: rat, human
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin embedded rat colon.; indirect immunofluorescence: 2.5-5 μg/mL using HeLa cells.; western blot: 1.5-3.0 μg/mL using using lysates of Jurkat cells.
• UniProt accession no.: P35637
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FUS(2521) ; rat ... Fus(317385)

Description

General description

Fused in sarcoma (FUS) is a component of heterogeneous nuclear ribonucleoprotein (hnRNP) complex is a DNA/ RNA binding protein. FUS gene is mapped to human chromosome 16p11.2 and is located predominantly in the nucleus.

Specificity

Anti-FUS specifically recognizes human and rat FUS.

Immunogen

synthetic peptide corresponding to the N-terminal region of human FUS isoform 1, conjugated to KLH. The corresponding sequence is identical in human FUS isoforms 2 and 3, and highly conserved (single amino acid insertion) in mouse and rat FUS.

Application

Anti-FUS antibody produced in rabbit has been used in:

• immunohistochemistry
• immunoblotting
• immunofluorescence

Biochem/physiol Actions

Fused in sarcoma (FUS) plays regulatory roles in transcription, RNA splicing and transport and is implicated in multiple diseases. FUS also called translocation in liposarcoma or Tumor lysis syndrome (TLS), plays a key role in DNA repair and transcriptional regulation. Chromosomal translocation of FUS/TLS is found in human cancers and results in the production of oncogenic FUS fusion proteins. FUS is a component of inclusion bodies in patients with Huntington′s disease (HD) and spinocerebellar ataxias (SCA1) and SCA3. Mutations in the FUS gene have been identified in amyotrophic lateral sclerosis (ALS), frontotemporal lobar degeneration (FLTD) and familial amyotrophic lateral sclerosis (FALS). The majority of the FUS mutations have been identified in the C-terminal nuclear localization signal (NLS). Pathological FUS inclusions are mostly found in the cytosol of neurons and glial cells.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable