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S8820

Sigma-Aldrich

Protease Inhibitor Cocktail

SIGMA FAST, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, For General Use

Synonym(s):

Protease Inhibitor Tablets, protease inhibitor

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

product name

SIGMAFAST™ Protease Inhibitor Tablets, For General Use

biological source

bovine lung
bovine milk

form

tablet

solubility

water: 1 tablet/100mL, clear to hazy, colorless

storage temp.

2-8°C

Related Categories

General description

SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract.
The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

SIGMAFAST Protease Inhibitor Tablets has been used:
  • to wash raw semen for membrane protein analysis
  • in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
  • as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
  • as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1ß1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)

These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.

Features and Benefits

  • Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
  • Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
  • Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
  • Tested and optimized for general use: proven performance and reliability for a wide range of applications.

Components

AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM

Quantity

One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.

Physical form

Tablet that is water soluble.

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

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Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H314,H335

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Molecular & cellular proteomics : MCP, 11(11), 1140-1155 (2012-07-31)
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The dopamine oxidation product cysteinyl-dopamine has attracted attention as a contributor to the death of dopaminergic neurons in Parkinson's disease. Treatment of cysteinyl-dopamine with hypochlorite yields an even more cytotoxic product. This product has potent redox-cycling activity and initiates production
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La Rocca C, et al.
Journal of biological and chemical chronicles, 77, 39-46 (2017)
Heather M Brechbuhl et al.
The American journal of pathology, 179(1), 367-379 (2011-06-28)
The purpose of this study was to determine whether β-catenin regulates basal cell fate determination in the mouse trachea. Analysis of TOPGal transgene reporter activity and Wnt/β-catenin pathway gene expression suggested a role for β-catenin in basal cell proliferation and
Giedrius Steponaitis et al.
Oncology letters, 12(5), 3305-3311 (2016-12-03)
Astrocytomas are one of the most common brain tumours; however, the current methods used to characterize these tumours are inadequate. The establishment of molecular markers may identify variables required to improve tumour characterization and subtyping, and may aid to specify
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Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

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Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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