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S5150

Sigma-Aldrich

Sodium chloride solution

5 M in H2O, BioReagent, for molecular biology, suitable for cell culture

Synonym(s):

Halite, NaCl, sodium chloride

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About This Item

Linear Formula:
NaCl
CAS Number:
Molecular Weight:
58.44
Beilstein:
3534976
EC Number:
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

sterility

0.2 μm filtered

product line

BioReagent

form

liquid

concentration

5 M in H2O

technique(s)

ChIP: suitable
DNA extraction: suitable
cell culture | mammalian: suitable

impurities

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected
<1.0 EU/mL Endotoxin level
<16 cfu/mL Bioburden

pH

6.0-8.0

density

1.190 g/cm3

anion traces

phosphate (PO43-): ≤5 ppm

cation traces

K: ≤50 ppm
heavy metals (as Pb): ≤5 ppm

suitability

suitable for Western blot
suitable for molecular biology

application(s)

agriculture
agriculture

foreign activity

DNAse, Exonuclease; RNAse; NICKase, Endonuclease; Protease, none detected

SMILES string

[Na+].[Cl-]

InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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Application

Sodium chloride solution has been used as a component of mycobacterial lysis buffer to extract DNA. It has also been used along with Dulbecco′s phosphate-buffered saline (DPBS) in the preparation of degassed buffer to elute plasma samples.

Preparation Note

Prepared in 18 megohm water.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

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