Skip to Content
Merck
All Photos(3)

Documents

MAK082

Sigma-Aldrich

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

ALDH Activity Assay Kit

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cardiovascular diseases; cancer; hematological disorder

storage temp.

−20°C

General description

Aldehyde dehydrogenases (ALDH) are a family of enzymes that catalyze the metabolism of exogenous and endogenous aldehydes, preventing the accumulation of potentially reactive and toxic aldehydes and their metabolites. In addition to their role in aldehyde metabolism, ALDH enzymes also play critical roles in other cellular processes such as cell proliferation, differentiation, and survival. Mutations in ALDH family members have been linked to numerous diseases such as heart disease, cataracts, and certain cancers. ALDH activity may also serve as a marker for certain stem cell populations including hematopoietic stem cells and certain cancer stem cells.

Application

Aldehyde Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the activity of aldehyde dehydrogenase.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of aldehyde dehydrogenase (ALDH) in a variety of samples including tissue and cells

Principle

The Aldehyde Dehydrogenase Activity Colorimetric Assay kit provides a simple and direct procedure for measuring NAD-dependent ALDH activity in a variety of samples. ALDH activity is determined by a coupled enzyme assay in which acetaldehyde is oxidized by ALDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the ALDH activity present. One unit of ALDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H314,H350

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The effects of antiviral treatment on breast cancer cell line.
Shaimerdenova M, et al.
Infectious Agents and Cancer, 12(1), 18-18 (2017)
Aldehyde Dehydrogenase Inhibitors: a Comprehensive Review of the Pharmacology, Mechanism of Action, Substrate Specificity, and Clinical Application.
Vindhya K, et al.
Pharmacological Reviews, 64(3), 520-539 (2012)
Bowen Gao et al.
Scientific reports, 11(1), 2408-2408 (2021-01-30)
Triple-negative breast cancer (TNBC) is associated with a high rate of early recurrence and distant metastasis, frequent development of therapeutic resistance, and a poor prognosis. There is a lack of targeted therapies for this aggressive subtype of breast cancer. Identifying
Aldehyde Dehydrogenases in Cellular Responses to Oxidative/electrophilic Stress
Surendra S, et al.
Free Radical Biology & Medicine, 56, 89-101 (2013)
Ting Liu et al.
Bioengineered, 12(1), 7143-7155 (2021-09-22)
Cancer stem cell (CSC) has been confirmed to trigger tumor occurrence and progression and CSC can develop strategies to maintain a lower reactive oxygen species (ROS) level compared to cancer cells. However, the mechanisms contributing to ROS homeostasis in CSC
View All Related Papers

Articles

Cancer Stem Cell Detection with AldeRed™ 588-A

AldeRed™ 588-A detects ALDH activity in cancer stem cells, aiding in cancer research.

Cancer Stem Cell Detection with AldeRed™ 588-A

AldeRed™ 588-A detects ALDH activity in cancer stem cells, aiding in cancer research.

Cancer Stem Cell Detection with AldeRed™ 588-A

AldeRed™ 588-A detects ALDH activity in cancer stem cells, aiding in cancer research.

Cancer Stem Cell Detection with AldeRed™ 588-A

AldeRed™ 588-A detects ALDH activity in cancer stem cells, aiding in cancer research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service