GW10817F

Anti-TAT antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-Protein Tat, Anti-Transactivating regulatory protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: chicken
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• manufacturer/tradename: Genway 15-288-10817F
• technique(s): western blot: suitable
• NCBI accession no.: NP_057853.1
• UniProt accession no.: P04608
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... tat(155871)

Description

Immunogen

Immunogen Sequence: GI # 9629358, sequence 1-86

Recombinant tat (human immunodeficiency virus 1)

Application

Anti-TAT antibody produced in chicken is suitable for western blotting at a working dilution of 1:500 and for cell staining at a working dilution of 1:200.

Biochem/physiol Actions

TAT (Transactivating regulatory protein) is a trans-activator of human immunodeficiency virus-1 (HIV-1) gene expression and is important for virus replication. It binds to the bulge loop RNA element called TAR, but can also associate with cellular transcription factors. Cyclin T binds with TAT and thereby enhances the affinity of TAT-TAR RNA interaction. Protein arginine methyltransferase-6 (PRMT6) is responsible for methylation of TAT and negatively affects the association between TAT and cyclin T. TAT is involved in the activation of nuclear factor κB (NFκB), a master regulator of pro-inflammatory genes. TAT binds with α5β1 and αvβ3 integrins, and causes vascular cell migration and invasion. At the neural plasma membrane, TAT induces formation of a complex involving the low-density lipoprotein receptor-related protein (LRP), postsynaptic density protein-95 (PSD-95), N-methyl-d-aspartic acid (NMDA) receptors and neuronal nitric oxide synthase (nNOS). This complex is responsible for apoptosis in neurons and astrocytes, causing neurologic dysfunction in AIDS patients.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves