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CL4B200

Supelco

Sepharose CL-4B Size Exclusion Resin

Cross-linked, 90-350 mesh

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12000000
NACRES:
SB.52

product name

Sepharose CL-4B, Cross-linked

Quality Level

form

beads

technique(s)

LPLC: suitable

matrix

Agarose

matrix active group

polymer

bead diameter

40-165 μm

pore size

30,000-5,000,000 fractionation range (Dextrans)
60,000-20,000,000 fractionation range (Globular proteins)

separation technique

size exclusion (SEC)

storage temp.

2-8°C

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General description

CL4B200-1L′s update product number is GE17-0150-01

Application

  • Molecular size distribution assessment of Haemophilus influenzae vaccine containing lactose by HPAEC-PAD and colorimetric assays: This study used Sepharose CL-4B in a methodology assessing molecular size distribution in vaccine formulations, indicating its application in quality control and development processes in vaccine production (Nadim AH et al., 2022).
  • Camel hydatidosis diagnostic kit: optimization of turnip and horseradish peroxidase conjugates using glutaraldehyde method: This research mentions the use of Sepharose CL-4B in the development of diagnostic kits, suggesting its role in enhancing diagnostic assay development through effective enzyme conjugation techniques (Kandil OM et al., 2020).
  • Pectin from leaves of birch (Betula pendula Roth.): Results of NMR experiments and hypothesis of the RG-I structure: Sepharose CL-4B was utilized in the structural elucidation of pectins from birch leaves, underscoring its importance in the structural study of polysaccharides which is essential for understanding their biological functions and industrial applications (Golovchenko VV et al., 2022).

Legal Information

Sepharose is a trademark of Cytiva

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Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F

Flash Point(C)

38 - 43 °C


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S Cochet et al.
Journal of chromatography. A, 663(2), 175-186 (1994-03-11)
A range of substances were screened to find eluents for human immunoglobulin G (IgG) which are retained with a strong affinity by immobilized Drimarene Rubine R/K-5BL. The strong affinity of IgG for the dye is partly due to the presence
M M Diogo et al.
Journal of chromatography. A, 1006(1-2), 137-148 (2003-08-27)
The effect of salt and temperature on the interaction of homo-oligonucleotides with a derivatized Sepharose CL-6B hydrophobic support has been explained by the application of two different models: the solvophobic theory and the preferential interaction analysis. It has been shown
Immunoadsorbents for clinical use: ex vivo immunoglobulin E removal in allergy.
V D Gorchakov et al.
Journal of chromatography, 563(1), 166-171 (1991-01-18)
M Hamai et al.
Comparative biochemistry and physiology. B, Comparative biochemistry, 95(2), 255-259 (1990-01-01)
1. Tryptic cleavage of fish myosin subfragment-1 (S-1) revealed its similar substructure of heavy chain to that of rabbit S-1. 2. The structural stability of fish S-1 was studied by thermal denaturation method, and a rapid polymerization of inactivated fish
N Ubrich et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 24(1), 65-75 (1996-01-01)
Immunoadsorption is an application of affinity chromatography, as a therapeutic method to specifically deplete biological fluids such as blood plasma from proteins in excess, or to extract a biomolecule from a complex mixture. However, the leakage of small amounts of

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