01494

Nancy-520

suitable for RT-PCR, ≥95.0% (HPCE)

• EC Number: 200-664-3
• UNSPSC Code: 12171500
• NACRES: NA.32

Properties

• Quality Level: 100
• Assay: ≥95.0% (HPCE)
• form: liquid
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• solubility: DMSO: soluble
• fluorescence: λ_ex 520 nm; λ_em 554 nm in TE buffer; DNA
• greener alternative category: Aligned,
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product was Designed for Safer Chemistry

Application

Nancy-520 is a fluorescent stain for dsDNA on agarose electrophoresis gels, with higher sensitivity than ethidium bromide and an easy, fast, and robust staining procedure. It is provided as a 5000x stock solution (500 μl), which is sufficient for 50 agarose gels. The limit of detection is 0.5 ng/band of dsDNA.
In addition, Nancy-520 can be used to determine dsDNA concentrations in solution, with a linear range between 0 - 2 μg/ml of DNA. Nancy 520

Nancy-520 has been used successfully in PCR (Real time-PCR) applications.
Nancy 520

Greener alternative to Ethidium bromide.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 188.6 °F - closed cup - Solvent
• Flash Point(C): 87 °C - closed cup - Solvent
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves