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M8640

Sigma-Aldrich

1-Methoxy-5-methylphenazinium methyl sulfate

≥95% purity , powder

Synonym(s):

1-Methoxyphenazine methosulfate

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About This Item

Empirical Formula (Hill Notation):
C15H16N2O5S
CAS Number:
65162-13-2
Molecular Weight:
336.36
EC Number:
265-579-6
MDL number:
MFCD00040648
UNSPSC Code:
12171500
PubChem Substance ID:
329818065
NACRES:
NA.47

product name

1-Methoxy-5-methylphenazinium methyl sulfate, ≥95%

Quality Level

200

Assay

≥95%

form

powder

composition

Nitrogen: ≥ 7.9%

color

red to brown

solubility

H2O: 1 mg/mL, clear, red

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

COS([O-])(=O)=O.COc1cccc2[n+](C)c3ccccc3nc12

InChI

1S/C14H13N2O.CH4O4S/c1-16-11-7-4-3-6-10(11)15-14-12(16)8-5-9-13(14)17-2;1-5-6(2,3)4/h3-9H,1-2H3;1H3,(H,2,3,4)/q+1;/p-1

InChI key

MASUWVVNWALEEM-UHFFFAOYSA-M

General description

1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) is a photochemically stable electron-transport mediator between NAD(P)H and tetrazolium dyes. The reduced form of MPMS converts tetrazolium salt to purple precipitate formazan.It is a very stable and non-enzymic electron carrier that mediates electron transfer by dehydrogenases in vitro to various electron acceptors such as tetrazolium dyes or the electrode of an enzymic electric cell.

Application

1-Methoxy-5-methylphenazinium methyl sulfate has been used as a component of buffers or solutions for the assay of lactate dehydrogenase and muscle succinate dehydrogenase.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 2

Target Organs

Nervous system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCM0974
MKCL6166
MKCL2964
MKCK4744
MKCJ5976

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Early life exposure to chronic intermittent hypoxia causes upper airway dilator muscle weakness, which persists into young adulthood
McDonald FB, et al.
Experimental Physiology, 100(8), 947-966 (2015)
An optimized lactate dehydrogenase release assay for screening of drug candidates in neuroscience.
Kaja S, et. al.
Journal of Pharmacological and Toxicological Methods, 73, 1-1 (2015)
Kuniyo Inouye et al.
Journal of biochemistry, 131(1), 97-105 (2002-01-05)
A synchronous enzyme-reaction system using water-soluble formazan and a non-enzymatic electron mediator was developed and applied to an enzyme immunoassay (EIA). The reaction system consists of four steps: (I) dephosphorylation of NADP(+) to produce NAD(+) by alkaline phosphatase (ALP), (II)
Early life exposure to chronic intermittent hypoxia causes upper airway dilator muscle weakness, which persists into young adulthood.
McDonald FB, et. al.
Experimental Physiology, 100, 947-947 (2015)
Debbie-Jane G Scarlett et al.
Biochimica et biophysica acta, 1708(1), 108-119 (2005-05-11)
Reduction of the cell-impermeable tetrazolium salt WST-1 has been used to characterise two plasma membrane NADH oxidoreductase activities in human cells. The trans activity, measured with WST-1 and the intermediate electron acceptor mPMS, utilises reducing equivalents from intracellular sources, while
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