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Key Documents

FCABS352A4

Sigma-Aldrich

Milli-Mark® Anti-Nanog-Alexa Fluor 488 Antibody, NT

Milli-Mark®, from rabbit

Synonym(s):

Homeobox transcription factor Nanog, Nanog homeobox

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NANOG(79923)

Related Categories

General description

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).

Specificity

Antibody recognizes NANOG.

Immunogen

Epitope: N-terminus
Linear peptide from mouse Nanog.

Application

Milli-Mark Anti-Nanog-Alexa Fluor 488 Antibody, N-terminus is an antibody against Nanog-Alexa Fluor 488 for use in FC.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Quality

Evaluated by flow cytometry using 2102 Ep cells

Target description

34 kDa Calculated

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal IgG conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
2102 Ep cells

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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