AB9864R
Anti-NMDAR1 Antibody, clone 1.17.2.6, rabbit monoclonal
clone 1.17.2.6, from rabbit, unconjugated
Synonym(s):
N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMDA receptor 1, glutamate [NMDA] receptor subunit zeta 1, glutamate receptor, ionotropic, N-methyl D-aspartate 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1.17.2.6, monoclonal
species reactivity
rat, mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GRIN1(2902)
mouse ... Grin1(14810)
rat ... Grin1(24408)
General description
N-methyl-D-aspartate receptor subunit NR1 (NMDAR1) is a multi-pass membrane protein belonging to the glutamate-gated ion channel family and is one of two subunits comprising the tetrameric protein NMDAR localized throughout the brain at excitatory synapses. NMDAR1 is mediated by glycine and is a critical component to excitotoxicity, learning, memory acquisition, synaptic plasticity, and synaptogenesis. It also plays a regulatory role for neuronal functions involving glutamate neurotransmission and the targeting of NMDA receptors on the cell surface. As a glutamate-gated ion channel, NMDAR1 is highly calcium permeable and possesses sensitivity to magnesium in a voltage-dependent manner.
Immunogen
Epitope: Recognizes NMDAR1 at the C-terminus
Linear peptide corresponding to human NMDAR1.
Application
Anti-NMDAR1 Antibody, clone 1.17.2.6 detects level of NMDAR1 & has been published & validated for use in WB, IH.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Western Blot Analysis: 0.1 µg/mL of this antibody from a previous lot detected NMDAR1 on 10 µg of mouse microsomal brain tissue lysate.
Immunohistochemistry Analysis: 1:250 dilution of this antibody from a previous lot detected NMDAR1 in rat cerebellum tissue.
Immunohistochemistry Analysis: 1:250 dilution of this antibody from a previous lot detected NMDAR1 in rat cerebellum tissue.
Quality
Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected NMDAR1 on 10 µg of rat brain tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected NMDAR1 on 10 µg of rat brain tissue lysate.
Target description
~120 kDa observed
Physical form
Protein A
Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide and 40% Glycerol.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Rat brain tissue lysate
Rat brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Mikel Pérez-Rodríguez et al.
Cerebral cortex (New York, N.Y. : 1991), 29(8), 3266-3281 (2018-09-01)
Critical periods of synaptic plasticity facilitate the reordering and refining of neural connections during development, allowing the definitive synaptic circuits responsible for correct adult physiology to be established. Presynaptic spike timing-dependent long-term depression (t-LTD) exists in the hippocampus, which depends
Juan Hong et al.
Neural plasticity, 2018, 3989201-3989201 (2018-08-21)
Cochlear inner hair cell (IHC) ribbon synapses play an important role in sound encoding and neurotransmitter release. Previous reports show that both noise and aminoglycoside exposures lead to reduced numbers and morphologic changes of synaptic ribbons. In this work, we
Mirko Feldmann et al.
Cerebral cortex (New York, N.Y. : 1991), 29(2), 892-905 (2018-12-12)
Although by adulthood cortical structures and their capacity for processing sensory information have become established and stabilized, under conditions of cortical injury, or sensory deprivation, rapid reorganization occurs. Little is known as to the impact of this kind of adaptation
Yasuhito Watanabe et al.
Frontiers in molecular neuroscience, 8, 87-87 (2016-01-23)
N-methyl-D-aspartate receptors (NMDARs) in all hippocampal areas play an essential role in distinct processes of memory formation as well as in sustaining cell survival of postnatally generated neurons in the dentate gyrus (DG). In contrast to the beneficial effects, over-activation
Emilia H Moscato et al.
Annals of neurology, 76(1), 108-119 (2014-06-12)
A severe but treatable form of immune-mediated encephalitis is associated with antibodies in serum and cerebrospinal fluid (CSF) against the GluN1 subunit of the N-methyl-D-aspartate receptor (NMDAR). Prolonged exposure of hippocampal neurons to antibodies from patients with anti-NMDAR encephalitis caused
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