100600C

E. coli Extract Polar

Avanti Research^™ - A Croda Brand

• Synonym(s): E. coli Polar Lipid Extract
• CAS Number: 1240502-50-4
• UNSPSC Code: 12352211
• NACRES: NA.25

Properties

• form: chloroform solution
• packaging: pkg of 2 × 20 mL (100600C-1g); pkg of 1 × 4 mL (100600C-100mg); pkg of 5 × 4 mL (100600C-500mg)
• manufacturer/tradename: Avanti Research^™ - A Croda Brand
• concentration: 25 mg/mL (100600C-100mg); 25 mg/mL (100600C-1g); 25 mg/mL (100600C-500mg)
• lipid type: lipid extracts
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.

Application

E. coli Extract Polar is suitable to prepare small unilamellar vesicles, to mimic the surface of the E. coli inner membrane.

E. coli extract polar has been for the preparation of liposomes.

E. coli Extract Polar has been used:

• in unilamellar liposomes preparation for kinetic studies
• in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies
• in the reconstitution of molybdate transport protein (MolBC) for continuous wave-electron paramagnetic resonance studies

Biochem/physiol Actions

E.coli Polar lipid extracts comprise phosphatidylethanolamine as major lipid component. The minor components include the phosphatidylglycerol (PG) and cardiolipin. The leaflets of cytoplasmic membrane and inner leaflet of outer membrane are the source of E. coli lipid extract. It is used for reconstituting membrane proteins and forms bilayer by vesicle fusion.

Packaging

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100600C-1g)

5 mL Clear Glass Sealed Ampule (100600C-100mg)

5 mL Clear Glass Sealed Ampule (100600C-500mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H302,H315,H319,H331,H336,H351,H361d,H372,H412
• Precautionary Statements: P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3
• Target Organs: Central nervous system, Liver,Kidney
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 3
• Flash Point(F): does not flash
• Flash Point(C): does not flash