Skip to Content
Merck
All Photos(1)

Documents

G005650

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] 100 mM ammonium phosphate monobasic, pH 7.0 with ammonium hydroxide; [B] water; [C] acetonitrile
gradient: 10% A constant; 85 to 25% B, 5 to 65% C in 6 min; 25% B, 65% C held for 5 min
flow rate: 0.3 mL/min
column temp.: 40 °C
detector: UV, 254 nm
injection: 5 μL
sample: 20 μg/mL each in water

suitability

application for HPLC

application(s)

food and beverages

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Tartrazine
    Amaranth
    Indigo carmine
    New Coccine
    Sunset Yellow FCF
    Allura Red AC
    Fast Green FCF
    Erioglaucine disodium salt
    Erythrosin B sodium salt
    Phloxine B
    Rose bengal
    See All (11)

analytical column

Product No.
Description
Pricing

standard

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service