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P7874

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone DO-2, purified from hybridoma cell culture

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DO-2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~53 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using total cell extract of A431 cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

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General description

Monoclonal Anti-p53 (mouse IgG1 isotype) is derived from the hybridoma DO-2 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53.
p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Immunogen

recombinant human wild type p53. The antibody epitope resides between amino acids 10-16 of human p53.

Application

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for DNase I footprint, immunoprecipitation and western blotting.

Biochem/physiol Actions

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.
p53 is phosphorylated at multiple sites by several protein kinases in response to DNA damage. It is essential for the maintenance of the non-tumorigenic phenotype of cells. Phosphorylation of p53 at Ser15 by ataxia telangiectasia mutate (ATM), ataxia telangiectasia and Rad3-related protein (ATR), and DNA-dependent protein kinase (DNA-PK) leads to a reduced interaction with its negative regulator MDM2 and accumulation of p53 protein. Checkpoint kinase 2 (Chk2) and Chk1 can phosphorylate p53 at Ser20, which enhances its activity, tetramerization, and stability. Elevation of p53 protein induces the transcriptional activation of multiple genes, including cyclin-dependent kinase inhibitor 1 (p21waf1).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
ATM mediates phosphorylation at multiple p53 sites, including Ser46, in response to ionizing radiation
Saito S, et al.
The Journal of biological chemistry, 277(15), 12491-12494 (2002)
Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
Augmentation of Radiation-Induced Apoptosis by ellagic acid
Sushma M
Cancer Investigation, 2, 323?330-323?330 (2010)
p53 C-terminal phosphorylation by CHK1 and CHK2 participates in the regulation of DNA-damage-induced C-terminal acetylation
Ou YH, et al.
Molecular Biology of the Cell, 16(4), 1684-1695 (2005)

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