N9162

Neocarzinostatin

from Streptomyces carzinostaticus, ≥90% (SDS-PAGE), solution

• Synonym(s): Holoneocarzinostatin, NCS, NSC-69856, Zinostatin
• CAS Number: 9014-02-2
• MDL number: MFCD01778130
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Product Name: Neocarzinostatin from Streptomyces carzinostaticus, ≥90% (SDS-PAGE), ~0.5 mg/mL
• Quality Level: 200
• Assay: ≥90% (SDS-PAGE)
• form: solution
• concentration: ~0.5 mg/mL
• antibiotic activity spectrum: neoplastics
• Mode of action: DNA synthesis | interferes
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: N([C@H]1[C@H](O[C@@H]([C@@H]([C@@H]1O)O)C)O[C@H]2[C@@H](C=C5C2CC#C[C@H]6O[C@]6(C#C5)[C@@H]7OC(=O)OC7)OC(=O)c3c4c(c(cc(c4)OC)C)ccc3O)C
• InChI: 1S/C35H35NO12/c1-16-12-19(42-4)14-22-20(16)8-9-23(37)27(22)32(40)45-24-13-18-10-11-35(26-15-43-34(41)46-26)25(48-35)7-5-6-21(18)31(24)47-33-28(36-3)30(39)29(38)17(2)44-33/h8-9,12-14,17,21,24-26,28-31,33,36-39H,6,15H2,1-4H3/t17-,21?,24-,25-,26-,28-,29+,30-,31-,33-,35+/m1/s1
• InChI key: BLXZMHNVKCEIJX-PNKAXBHOSA-N

Description

Application

Neocarzinostatin from Streptomyces carzinostaticus has been used:

• as a DNA damaging agent to treat human fibroblast GM0637 cells[1]
• as a radiomimetic antibiotic to induce DNA damage[2]
• in combination with Vγ2Vδ2 T cells to determine the cytotoxicity in ovarian cancer cells[3]

Biochem/physiol Actions

Neocarzinostatin is a protein-small molecule complex composed of an enediyne chromophore tightly bound to a 113 amino acid single chain protein. The complex possesses antiproliferative and antitumor activity. The chromophore is the active compound, which is responsible for DNA cleavage; while the apoprotein stabilizes and regulates the availability of the labile chromophore. NCS chromophore is bound non-covalently in a cleft of the binding protein and is dissociable. Upon addition of a thiol, the chromophore forms a highly reactive biradical species that can induce sequence-specific single and double strand breaks in DNA. Neocarzinostatin inhibits DNA synthesis and possesses antitumor activity in various human and animal tumors. NCS inhibits cellular proliferation by inducing G₂ cell cycle arrest and apoptosis in both human papillomavirus (HPV) positive and negative cell lines.

Neocarzinostatin is a radiomimetic antibiotic substance.[2]

Protein-small molecule complex composed of an enediyne chromophore tightly bound to a 113-amino acid single chain protein. Chemotherapeutic agent, which inhibits DNA synthesis, possesses antitumor activity, and induces apoptosis.

Physical form

Supplied as 100 μg (~9 nmoles) in a solution containing 20 mM MES buffer, pH 5.5.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves