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G4896

Sigma-Aldrich

Monoclonal Anti-Gelsolin antibody produced in mouse

clone GS-2C4, ascites fluid

Synonym(s):

Gelsolin Detection Antibody, Mouse Anti-Gelsolin

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GS-2C4, monoclonal

contains

15 mM sodium azide

species reactivity

bovine, pig, human, rabbit

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using heat-induced antigen retrieval sections
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using human plasma

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GSN(2934)

General description

Monoclonal Anti-Gelsolin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes of an immunized mouse. Gelsolin (GSN) is encoded by the gene mapped to human chromosome 9q33.2. GSN is a calcium dependent actin binding protein. It has been shown to exist in at least two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin has also been called as brevin. GSN is characterized with six domains G1-G6 at N-terminal end, each domain contains Ca2+ binding site.

Specificity

The antibody is specific for an epitope located on the 47 kDa peptide derived from a chymotryptic cleavage of gelsolin. The antibody reacts with plasma and cytoplasmic gelsolin. An additional application for this antibody is immunoaffinity chromatography, for purification of gelsolin, and for detection of gelsolin-actin complexes in cell extracts.

Immunogen

human plasma gelsolin.

Application

Monoclonal Anti-Gelsolin antibody produced in mouse has been used in immunoprecipitation and immunohistochemical staining.

Biochem/physiol Actions

Gelsolin (GSN) is a potent modulator of actin filament length and gelation. It binds to the plus′ ends of actin monomers and filaments, preventing monomer exchange. GSN plays a vital role in regulation of actin dynamics. Mutations in this gene cause familial amyloidosis Finnish type (FAF) characterized by cranial neuropathy and lattice corneal dystrophy. Plasma gelsolin is used as a biochemical marker for acute rheumatic carditis.

Target description

Gelsolin is an actin-severing protein found in mammalian cells and blood plasma. Has tumor suppressor activity in several cancer cell lines and in nude mice.

Other Notes

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200750 Anti-Gelsolin antibody, Mouse Monoclonal
clone GS-2C4, purified from hybridoma cell culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Naomi Walsh et al.
Journal of proteomics, 71(5), 561-571 (2008-10-14)
Conditioned medium (CM) from clonal sub-populations of the pancreatic cancer cell line, MiaPaCa-2 with differing invasive abilities, were examined for their effect on in vitro invasion. Conditioned medium from Clone #3 (CM#3) strongly promoted invasion, while CM from Clone #8
Robert Bucki et al.
Journal of immunology (Baltimore, Md. : 1950), 181(7), 4936-4944 (2008-09-20)
The various functions of gelsolin in extracellular compartments are not yet clearly defined but include actin scavenging and antiinflammatory effects. Gelsolin was recently reported to bind endotoxin (LPS) from various Gram-negative bacteria with high affinity. In this study we investigate
Elizabeth S Harris et al.
The Journal of biological chemistry, 279(19), 20076-20087 (2004-03-03)
Formins are a conserved class of proteins expressed in all eukaryotes, with known roles in generating cellular actin-based structures. The mammalian formin, FRLalpha, is enriched in hematopoietic cells and tissues, but its biochemical properties have not been characterized. We show
Masaki Ikeda et al.
Journal of the neurological sciences, 252(1), 4-8 (2006-11-14)
Familial amyloid polyneuropathy (FAP: type IV), known as familial amyloidosis of the Finnish type (FAF), is very rare and reported only in a few countries. The gelsolin mutation G654A is most frequent causative gene in FAF family. The clinical phenotype
ATF3 suppresses metastasis of bladder cancer by regulating gelsolin-mediated remodeling of the actin cytoskeleton
Yuan X, et al.
Cancer research, 73(12), 3625-3637 (2013)

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