Skip to Content
Merck
All Photos(3)

Documents

CMC0014

Sigma-Aldrich

BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

Synonym(s):

Competent E. coli cells

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
41106202
NACRES:
NA.85

product name

BL21(DE3) Chemically Competent Cells, for protein expression

biological source

Escherichia coli

grade

for molecular biology

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

The E. coli strain BL21 (DE3) has been used:

  • to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
  • BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
  • to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.

Features and Benefits

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Components

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

related product

Product No.
Description
Pricing

L3022

LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L2897

LB Broth with agar (Lennox), Highly-referenced microbial growth powder medium with Agar, low salt, suitable for salt-sensitive E. coli culture.

L5542

LB Agar Plates, pre-poured LB agar plates for E. coli

B11001

EnPresso® B, Growth system for expressing protein in bacteria

H9950

Hanahan′s Broth (SOB Medium), Liquid microbial growth medium

CMC0015

BL21(DE3) pLysE Chemically Competent Cells, for protein expression

CMC0017

OverExpress™ C41(DE3) Chemically Competent Cells, for the highest protein expression

CMC0023

CONTROLLER SIG10 Chemically Competent Cells, for controlled protein expression

CMC0024

CONTROLLER BL21(DE3) Chemically Competent Cells, for controlled protein expression

CMR0001

Recovery Medium for Expression, for use with competent cells

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

David B Sauer et al.
eLife, 9 (2020-09-02)
Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters
A validated antibody panel for the characterization of tau post-translational modifications.
Ebru E, et al.
Mol. Neurodegener., 12(1), 87-87 (2017)
Yasaman Damestani et al.
Lasers in surgery and medicine, 48(8), 782-789 (2016-10-19)
The development and feasibility of a novel nanocrystalline yttria-stabilized-zirconia (nc-YSZ) cranial implant has been recently established. The purpose of what we now call "window to the brain (WttB)" implant (or platform), is to improve patient care by providing a technique
Romana Koszagova et al.
Microbial cell factories, 17(1), 139-139 (2018-09-05)
Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction
Gints Kalnins et al.
Nature communications, 11(1), 388-388 (2020-01-22)
Bacterial microcompartments (BMCs) are prokaryotic organelles consisting of a protein shell and an encapsulated enzymatic core. BMCs are involved in several biochemical processes, such as choline, glycerol and ethanolamine degradation and carbon fixation. Since non-native enzymes can also be encapsulated
View All Related Papers

Related Content

BL21 Chemically Competent Cells Overview

BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

BL21 Chemically Competent Cells Overview

BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

BL21 Chemically Competent Cells Overview

BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

BL21 Chemically Competent Cells Overview

BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service