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B1059

Sigma-Aldrich

Bcl-w (Minus C-Terminus) human

>95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

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About This Item

MDL number:
MFCD03846039
UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

200

Assay

>95% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

18 kDa by SDS-PAGE

UniProt accession no.

Q92843

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... BCL2L2(599)

General description

This recombinant Bcl-w lacks the mitochondrial targeting sequence but maintains the ability to neutralize pro-apoptotic Bcl-2 family members.

Biochem/physiol Actions

Like Bcl-2 and Bcl-x, the Bcl-w protein promotes cell survival, in contrast to other close homologues, Bax and Bak, which facilitate cell death. Bcl-w is an anti-apoptotic member of the Bcl-2 family that prevents release of cytochrome c from the mitochondrial intermembrane space into the cytosol. It is required for normal sperm maturation. Natural Bcl-w contains a C-terminal mitochondrial targeting sequence.

Physical form

Supplied as a 0.2 μm filtered solution in 25 mM HEPES, pH 7.5, 0.1 M KCl, and 10% glycerol.

Analysis Note

Measured by its ability to inhibit caspase-8-cleaved human BID-mediated release of cytochrome c from isolated mouse liver mitochondria using ELISA.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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W Yan et al.
Molecular endocrinology (Baltimore, Md.), 14(5), 682-699 (2000-05-16)
Bcl-w, a prosurvival member of the Bcl-2 family, is essential for spermatogenesis. However, the mechanisms by which Bcl-w participates in the regulation of apoptosis in the testis are largely unknown. To explore the potential role of Bcl-w in the regulation
The proto-oncogene Bcl-2 and its role in regulating apoptosis.
G Kroemer
Nature medicine, 3(6), 614-620 (1997-06-01)
Sajid Khan et al.
Nature medicine, 25(12), 1938-1947 (2019-12-04)
B-cell lymphoma extra large (BCL-XL) is a well-validated cancer target. However, the on-target and dose-limiting thrombocytopenia limits the use of BCL-XL inhibitors, such as ABT263, as safe and effective anticancer agents. To reduce the toxicity of ABT263, we converted it
BCL-2 family members and the mitochondria in apoptosis.
A Gross et al.
Genes & development, 13(15), 1899-1911 (1999-08-13)
Yonghan He et al.
Nature communications, 11(1), 1996-1996 (2020-04-26)
Small molecules that selectively kill senescent cells (SCs), termed senolytics, have the potential to prevent and treat various age-related diseases and extend healthspan. The use of Bcl-xl inhibitors as senolytics is largely limited by their on-target and dose-limiting platelet toxicity.

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