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Key Documents

MAB394-100UG

Sigma-Aldrich

Anti-Dopamine β Hydroxylase-SAP Antibody, clone 4F10.2

clone 4F10.2, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

saporin

antibody product type

primary antibodies

clone

4F10.2, monoclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

immunolesioning: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

rat ... Dbh(25699)

Specificity

Dopamine b-HydroxylaseDBH is a single gene product that exists as a cytosolic enzyme, as a membrane protein and is secreted into the circulation (Lewis & Asnani, 1992). Because of its residence time as a plasma membrane-bound protein, it is able to be used as a targeting agent for neurons. The target neurons include postganglionic sympathetic neurons and all noradrenergic and adrenergic neurons of the central nervous system. As such anti-DBH-SAP can be used for the production of acute peripheral autonomic failure in a model for the human disease. It can also be used for the analysis of the behavioral effects of noradrenergic or adrenergic ablation. When injected intraventricularly, noradrenergic neurons of the locus coeruleus are eliminated (Wiley & Lappi, 1994). The antibody and immunotoxin undergo retrograde transport.

MAB308 is the unconjugated monoclonal.

Application

Detect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase-SAP Antibody, clone 4F10.2 validated for use in 0.
Immunotoxin
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Physical form

Saporin Conjugated. Sterile liquid. Provided in phosphate buffered saline. Contains no preservative.

Storage and Stability

The material should be stored at -80°C for up to six months under sterile conditions. Avoid repeated freeze-thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M J Picklo et al.
The Journal of pharmacology and experimental therapeutics, 275(2), 1003-1010 (1995-11-01)
Anti-dopamine beta-hydroxylase immunotoxin (DHIT) is an antibody-targeted noradrenergic lesioning tool comprised of a monoclonal antibody against the noradrenergic enzyme, dopamine beta-hydroxylase, conjugated to saporin, a ribosome-inactivating protein. Noradrenergic-neuron specificity and completeness and functionality of sympathectomy were assessed. Adult, male Sprague-Dawley
C C Wrenn et al.
Brain research, 740(1-2), 175-184 (1996-11-18)
The ability to create lesions of discrete neuronal populations is an important strategy for clarifying the function of these populations. The power of this approach is critically dependent upon the selectivity of the experimental lesioning technique. Anti-neuronal immunotoxins offer an
Carolina Rocha-Pereira et al.
Journal of pharmacological sciences, 122(2), 59-70 (2013-06-21)
In hypertension, vascular reactivity alterations have been attributed to numerous factors, including higher sympathetic innervation/adenosine. This study examined the modulation of adenosine receptors on vascular sympathetic nerves and their putative contribution to higher noradrenaline spillover in hypertension. We assessed adenosine
Soluble and membrane-bound forms of dopamine beta-hydroxylase are encoded by the same mRNA.
Lewis, E J and Asnani, L P
The Journal of Biological Chemistry, 267, 494-500 (1992)

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