MAB1569

Anti-Microglia Antibody, clone 25F9

clone 25F9, Chemicon^®, from mouse

• Synonym(s): Macrophages, Late State Inflammatory
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 25F9, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable
• isotype: IgG1
• shipped in: wet ice
• target post-translational modification: unmodified

Description

General description

ANTIGEN DISTRIBUTION:

Isolated cells: Absent on freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 day culture, also positive on some melanoma and carcinoma cell lines. Tissue sections: Kupffer cells, histiocytes (skin), macrophages of the thymus, in the germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma; synovial lining cells, tuberculoid leprosy; no expression in lepramatous leprosy.

Specificity

Human late stage inflammatory macrophages. The monoclonal is suitable for use on BAL (bronchial lavage fluids) and other lavages. The antigen recognized by MAB1569 is an 86 kDa protein (unreduced conditions), probably a glycoprotein on the cell surface and within the cytoplasm of mature macrophages. It is stable to formaldehyde fixation and paraffin embedding. Enzyme digestion is recommended. The antibody has also been reported to recognize microglia stem cells (X International Congress of Neuropathology, Stockholm, le 11 September,1986.PosterA-701).

Antigen distribution: absent from freshly isolated monocytes and other blood cells; present on 40-50% of human monocytes after 6-7 days in culture, also positive on some melanoma and carcinoma lines. In tissue sections, clone identifies Kupffer cells, histiocytes (skin), macrophages of the thymus, in te germinal centers of lymph nodes and spleen, in mamma carcinoma, melanoma, osteocarcinoma and gastric cancer; excema, sarcoidosis, BCG granuloma;synovial lining cells, tuberculoid leprosy, however no expression in lepramatous leprosy.

Species reactivity is seen in human, rhesus monkey, and pig, other species not tested.

Application

Anti-Microglia Antibody, clone 25F9 detects level of Microglia & has been published & validated for use in FC, IC, IH.

Immunohistochemistry: 1:50-1:200 using human tonsil cryosection. The antibody is reactive on formalin fixed / paraffin sections. Enzyme digestion (proteinase pretreatement) is recommended.

Immunocytochemistry

Optimal working dilutions must be determined by the end user.

Physical form

Format: Purified

Purified immunoglobulin from culture supernatant. Lyophilized from PBS, pH 7.2 containing 5 mg/mL BSA and 0.09% sodium azide. Reconstitute with 500 μL of sterile distilled water.

Storage and Stability

Maintain lyophilized material at 2-8°C for up to 6 months. After reconstitution maintain at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H312 + H332,H412
• Precautionary Statements: P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3