07-164
Anti-phospho-H2A.X (Ser139) Antibody
Upstate®, from rabbit
Synonym(s):
H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, vertebrates
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
phosphorylation (pSer139)
Gene Information
human ... HIST2H2AC(8338)
General description
Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
Specificity
Broad species cross-reactivity expected based on conservation of sequence homology.
Histone H2A.X phosphorylated at Ser139.
Immunogen
Epitope: a.a. 134-142
Application
Anti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.
Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.
Quality
Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.
Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.
Target description
15 kDa
Linkage
Replaces: MABE205
Physical form
Format: Purified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analysis Note
Control
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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