T9157
Tryptose Phosphate Broth
suitable for insect cell culture
Synonym(s):
Tryptose Broth
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About This Item
UNSPSC Code:
41106507
NACRES:
NA.73
Recommended Products
biological source
bovine milk
Quality Level
form
solid
technique(s)
cell culture | insect: suitable
shipped in
ambient
storage temp.
room temp
General description
Tryptose Phosphate Broth (TPB) comprises pyrimidine based components and is crucial for pyrimidine synthesis and electron transport chain in cells. In chick embryo fibroblast, it alleviates chloramphenicol inhibition.
Application
In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Tryptose Phosphate Broth has been used as a supplement:
- in Eagle′s minimum essential medium alpha for growing Vero cells
- for Vero-CCL81 cells in porcine epidemic diarrhea coronavirus propagation
- in Dulbecco modified Eagle medium in LR7cells for mouse hepatitis virus propagation
- in Glasgow minimum essential medium (GMEM) for Madin-Darby canine kidney (MDCK) cells.
Components
Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Tryptose phosphate broth improves Rickettsia felis replication in mammalian cells
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