Skip to Content
Merck
All Photos(1)

Key Documents

SRP3108

Sigma-Aldrich

Maspin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Protease inhibitor 5, SerpinB5

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

42.2 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SERPINB5(5268)

General description

Maspin (mammary serine protease inhibitor), also known as SERPINB5 (serpin family B member 5), was first identified in 1994. It is an epithelial-specific protein that belongs to the serine protease inhibitor superfamily known as serpin. It shows major expression in human mammary epithelial cells.
Recombinant human Maspin is a 42.2kDa non-glycosylated protein containing 375 amino acid residues.

Biochem/physiol Actions

Maspin (mammary serine protease inhibitor) functions as a tumor suppressor in various types of cancer, such as breast cancer, prostate cancer etc. Its expression in cancer is thought to be controlled by DNA methylation, and in breast cancer and glioma its expression is silenced by abnormal methylation. In tissues of pulmonary adenocarcinoma (AC) patients, the mRNA expression of maspin is significantly increased, and hence, maspin mRNA expression might have potential as a prognosis marker in AC. Study in two MIBC (muscle invasive bladder cancer) cell lines (T24 and 5637) shows that overexpression of maspin can increase cisplatin chemosensitivity. In MIBC patients receiving cisplatin based neoadjuvant chemotherapy, the expression of maspin correlates with the prognosis.
Recombinant human Maspin is a 42.2kDa non-glycosylated protein containing 375 amino acid residues.

Sequence

MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDIPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRLNKT DTKPVQMMNM EATFCMGNID SINCKIIELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Aberrant Maspin mRNA Expression is Associated with Clinical Outcome in Patients with Pulmonary Adenocarcinoma.
Lu M et al
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22, 134-139 (2016)
Maspin enhances cisplatin chemosensitivity in bladder cancer T24 and 5637 cells and correlates with prognosis of muscle-invasive bladder cancer patients receiving cisplatin based neoadjuvant chemotherapy.
Chen J et al
Journal of Experimental & Clinical Cancer Research, 35, 2-2 (2016)
Methylation-induced silencing of maspin contributes to the proliferation of human glioma cells.
Xu L et al
Oncology Reports, 36(1), 57-64 (2016)
Zhila Khalkhali-Ellis
Clinical cancer research : an official journal of the American Association for Cancer Research, 12(24), 7279-7283 (2006-12-26)
Maspin (mammary serine protease inhibitor) was identified in 1994 by subtractive hybridization analysis of normal mammary tissue and breast cancer cell lines. Subsequently, emerging evidence portrays maspin as a multifaceted protein, interacting with diverse group of intercellular and extracellular proteins
Chikako Takeda et al.
Diagnostic pathology, 9, 205-205 (2014-11-02)
Maspin is a 42 kDa protein known to act as a tumor suppressor. Although its function has not been fully elucidated, numerous reports have investigated the prognostic impact of maspin in patients with several types of cancer. However, there have

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service