PROTIA
ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit
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About This Item
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technique(s)
immunodepletion: suitable
storage temp.
2-8°C
General description
ProteoPrep® Immunoaffinity Albumin and IgG Depletion Kit might be a useful tool for novel biomarker discovery.
The ProteoPrep® Immunoaffinity medium in the prepacked spin columns is a mixture of two-beaded media containing recombinantly expressed, small single-chain antibody ligands, resulting in low non-specific binding and high capacity. The ProteoPrep Immunoaffinity Albumin and IgG Depletion Kit has been designed to specifically remove albumin and IgG from human serum (25-50μL) in preparation of samples for proteomics analysis, two-dimensional electrophoresis (2DE) or liquid chromatography (LC). This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum.
Application
ProteoPrep® Immunoaffinity Albumin & amp; IgG Depletion Kit has been used in:
- umbilical cord serum proteomics
- human plasma (HP) proteomics
- proteomic profile-based screening of potential protein biomarkers in the urine of patients with nephrotic syndrome
Features and Benefits
- High Capacity removes more interfering abundant proteins
- High Specifity ensures you see an accurate and more complete proteome
- Buffer systems are compatible with 2 dimensional electrophoresis so there are no intermediate time consuming steps that might result in protein loss
Legal Information
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Journal of diabetes research, 2018, 6165303-6165303 (2018-06-02)
Urinary proteome was analyzed and quantified by tandem mass tag (TMT) labeling followed by bioinformatics analysis to study diabetic nephropathy (DN) pathophysiology and to identify biomarkers of a clinical outcome. We included type 2 diabetic normotensive non-obese males with (n
Analytical and bioanalytical chemistry, 411(3), 777-786 (2018-11-25)
High- and low-density lipoproteins (HDL and LDL) are attractive targets for biomarker discovery. However, ultracentrifugation (UC), the current methodology of choice for isolating HDL and LDL, is tedious, requires large sample volume, results in sample loss, and does not readily
Journal of tissue engineering, 10, 2041731419845852-2041731419845852 (2019-05-21)
Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin-chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow
Frontiers in cell and developmental biology, 9, 650490-650490 (2021-06-01)
Human platelet lysate (hPL) is considered a valid substitute to fetal bovine serum (FBS) in the expansion of mesenchymal stromal cells (MSC), and it is commonly produced starting from intermediate side products of whole blood donations. Through freeze-thaw cycles, hPL
Gut, 67(3), 473-484 (2016-11-09)
To discover and confirm blood-based colon cancer early-detection markers. We created a high-density antibody microarray to detect differences in protein levels in plasma from individuals diagnosed with colon cancer <3 years after blood was drawn (ie, prediagnostic) and cancer-free, matched controls.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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