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P4902

Sigma-Aldrich

2-Phenylethyl β-D-thiogalactoside

≥98% (TLC)

Synonym(s):

PETG, Phenethyl β-D-thiogalactoside

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About This Item

Empirical Formula (Hill Notation):
C14H20O5S
CAS Number:
63407-54-5
Molecular Weight:
300.37
Beilstein:
20033
MDL number:
MFCD00057554
UNSPSC Code:
12352201
PubChem Substance ID:
24898569
NACRES:
NA.25

Quality Level

200

Assay

≥98% (TLC)

form

powder

optical activity

[α]/D -33.00 to -27.00°, c = 9.00-11.00 mg/mL in methanol

technique(s)

thin layer chromatography (TLC): suitable

color

white

solubility

methanol: 49.00-51.00 mg/mL, clear, colorless

storage temp.

2-8°C

SMILES string

OC[C@H]1O[C@@H](SCCc2ccccc2)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H20O5S/c15-8-10-11(16)12(17)13(18)14(19-10)20-7-6-9-4-2-1-3-5-9/h1-5,10-18H,6-8H2/t10-,11+,12+,13-,14+/m1/s1

InChI key

ZNAMMSOYKPMPGC-HTOAHKCRSA-N

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General description

2-Phenylethyl β-D-thiogalactoside is a cell-permable inhibitor of the reporter enzyme β-galactosidase.

Application

2-Phenylethyl β-D-thiogalactoside has been used in a study to assess subnanoliter enzymatic assays on microarrays. It has also been used in a study that investigated the integration of on-column immobilized enzyme reactor in microchip electrophoresis.

Other Notes

To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Philipp Angenendt et al.
Proteomics, 5(2), 420-425 (2005-02-09)
Many areas of research today are based on enzymatic assays most of which are still performed as enzyme-linked immunosorbent assays in microtiter plates. The demand for highly parallel screening of thousands of samples eventually led to a miniaturization and automation
R A Edwards et al.
Biochemistry, 29(49), 11001-11008 (1990-12-11)
The thermal denaturation of wild-type beta-galactosidase and two beta-galactosidases with substitutions at the active site was studied by kinetics, differential scanning calorimetry, electrophoresis, molecular exclusion chromatography, and circular dichroism. From the results, a model is developed for thermal denaturation of
Kyung-Han Lee et al.
European journal of nuclear medicine and molecular imaging, 31(3), 433-438 (2004-01-28)
There has recently been increasing interest in the development of radioprobes that specifically target proteins transcribed from expression of reporter genes of interest. The purpose of this study was to develop a radioprobe that targets one of the most widely
Todd Galbraith et al.
Biotechnology journal, 14(1), e1800306-e1800306 (2018-11-30)
There is a strong clinical need to develop small-caliber tissue-engineered blood vessels for arterial bypass surgeries. Such substitutes can be engineered using the self-assembly approach in which cells produce their own extracellular matrix (ECM), creating a robust vessel without exogenous
Pranav R H Joshi et al.
Molecular therapy. Methods & clinical development, 13, 279-289 (2019-03-20)
Despite numerous advancements in production protocols, manufacturing AAV to meet exceptionally high demand (1016-1017 viral genomes [VGs]) in late clinical stages and for eventual systemic delivery poses significant challenges. Here, we report an efficient, simple, scalable, robust AAV5 production process
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