CRISPRPL02
CRISPR GUS GAPDH Reporter Control for Dicots
About This Item
recombinant
expressed in E. coli
Quality Level
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)
CRISPR
selection
kanamycin
shipped in
dry ice
storage temp.
−20°C
General description
CRISPR Plant Cas9 products are intended for Agrobacterium-mediated plant transformation. The products are based on the type IIA CRISPR-Cas9 derived from Streptococcus pyogenes. The native Cas9 coding sequence is codon optimized for expression in monocots and dicots, respectively. The monocot Cas9 constructs contain a monocot U6 promoter for sgRNA expression, and the dicot Cas9 constructs contain a dicot U6 promoter.
Arabidopsis seedlings were germinated in 6 well tissue culture plates
The seedlings were infected with Agrobacterium which had the CRISPR plasmids with a GUS reporter. After 3-4 days of transfection the GUS expression was detected. b-glucuronidase (GUS) is an enzyme that hydrolyzes colorless glucuronides to yield colored product
Application
- To verify successful integration of T-DNA in plant genome
- GUS receptor wheat gGAPDH control for monocots for Agrobacterium mediated transformation
Features and Benefits
- Low cost, genome editing option compared to other methods.
- Easy to use
- Online ordering
- Ready to ship in 2 days
Components
Keep reagent tubes closed when not in use.
Practice aseptic lab technique to avoid DNase contamination.
Principle
Other Notes
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.
ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.
ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.
ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.
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